Nair Manju Ananthakrishnan, Shaik Khadar Vali, Kokkiligadda Adiseshu, Gorrela Harsha
Department of Oral and Maxillofacial Surgery, PAHER University, Udaipur, India.
Department of Biotech Engineering, Virchow Biotech Private Limited, Hyderabad, India.
J Med Life. 2020 Jul-Sep;13(3):418-425. doi: 10.25122/jml-2020-0044.
The study aimed to investigate whether a 3D printed beta-tricalcium phosphate (β-TCP) scaffold tethered with growth factors and fibrin glue implanted autologous bone marrow-derived mesenchymal stem cells would provide a 3D platform for bone regeneration resulting in new bone formation with plasticity. Twenty 3D printed β-TCP scaffolds, ten scaffolds engrained with osteogenic mesenchymal stem cells with fibrin glue (group A), and ten scaffolds used as a control group with β-TCP scaffold and fibrin glue inoculation only (group B) were included in the study. Cell infiltration, migration, and proliferation of human osteogenic stem cells on the scaffolds were executed under both static and dynamic culture conditions. Each scaffold was examined post culture after repeated changes in the nutrient medium at 2, 4 or 8 weeks and assessed for opacity and formation of any bone-like tissues macroscopic, radiographic, and microscopic evaluation. Significant changes in all the prerequisite parameters compiled with an evaluated difference of significance showing maxillofacial skeletal repair via tissue engineering by β-TCP scaffold and MSCs remains will be the most promising alternative to autologous bone grafts and numerous modalities involving a variety of stem cells, growth factors from platelet-rich fibrin.
该研究旨在调查植入自体骨髓间充质干细胞并与生长因子和纤维蛋白胶相连的3D打印β-磷酸三钙（β-TCP）支架是否能为骨再生提供一个3D平台，从而形成具有可塑性的新骨。该研究纳入了20个3D打印的β-TCP支架，其中10个支架用纤维蛋白胶植入成骨间充质干细胞（A组），另外10个支架仅用β-TCP支架和纤维蛋白胶接种作为对照组（B组）。在静态和动态培养条件下观察人成骨干细胞在支架上的细胞浸润、迁移和增殖情况。在2、4或8周反复更换营养培养基后对每个支架进行培养后检查，并通过宏观、影像学和显微镜评估对不透明度和任何骨样组织的形成进行评估。所有前提参数的显著变化与评估的显著性差异相结合，表明通过β-TCP支架和间充质干细胞进行组织工程修复颌面骨骼仍然是自体骨移植以及涉及多种干细胞、富含血小板纤维蛋白中的生长因子的众多方式最有前景的替代方法。
