Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India.
Colloids Surf B Biointerfaces. 2012 Jun 15;95:235-40. doi: 10.1016/j.colsurfb.2012.03.001. Epub 2012 Mar 20.
Leaf extract of Morinda citrifolia L. was assessed for the synthesis of silver nanoscale particles under different temperature and reaction time. Synthesized nanoscale (MCAgNPs) particles were confirmed by analysing the excitation of surface plasmon resonance (SPR) using UV-visible spectrophotometer at 420 nm. Further SEM, HRTEM analysis confirmed the range of particle size between 10 and 60 nm and SEAD pattern authorizes the face centered cubic (fcc) crystalline nature of the MCAgNPs. Fourier transform infrared spectrum (FTIR) of synthesized MCAgNPs confirms the presence of high amount of phenolic compounds in the plant extract which may possibly influence the reduction process and stabilization of nanoparticles. Further, inhibitory activity of MCAgNPs and plant extract were tested against human pathogens like Eschericia coli, Pseudomonas aeroginosa, Klebsiella pneumoniae, Enterobacter aerogenes, Bacillus cereus and Enterococci sp. The results indicated that the MCAgNPs showed moderate inhibitory actions against human pathogens than crude plant extract, demonstrating its antimicrobial value against pathogenic diseases.
桑枝提取物在不同温度和反应时间下被评估用于合成银纳米颗粒。通过在 420nm 处使用紫外可见分光光度计分析表面等离子体共振（SPR）的激发，来验证合成的纳米级（MCAgNPs）颗粒。进一步的 SEM、HRTEM 分析证实了粒径范围在 10nm 至 60nm 之间，SEAD 图谱授权 MCAgNPs 的面心立方（fcc）结晶性质。合成的 MCAgNPs 的傅立叶变换红外光谱（FTIR）证实了植物提取物中存在大量的酚类化合物，这可能会影响还原过程和纳米颗粒的稳定性。此外，MCAgNPs 和植物提取物对人类病原体如大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌、产气肠杆菌、蜡状芽孢杆菌和肠球菌的抑制活性进行了测试。结果表明，MCAgNPs 对人类病原体的抑制作用比粗提植物提取物温和，证明了其对致病疾病的抗菌价值。
