Urcan Adriana Cristina, Criste Adriana Dalila, Szanto Karina Ioana, Ștefan Razvan, Zahan Marius, Muscă Adriana Sebastiana, Focsan Monica, Burtescu Ramona Flavia, Olah Neli Kinga
Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, 400372 Cluj-Napoca, Romania.
Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania.
Pharmaceutics. 2023 Jun 23;15(7):1797. doi: 10.3390/pharmaceutics15071797.
Bee bread (BB) is a fermented mixture of bee pollen, is rich in proteins, amino acids, fatty acids, polyphenols, flavonoids, as well as other bioactive compounds, and is considered functional food for humans. In this study, we explored an innovative green synthesis of colloidal silver nanoparticles, using BB extracts as reducing and stabilizing agents. A preliminary chemical characterization of the BB extracts was conducted. The plasmonic response of the as-synthesized silver nanoparticles (BB-AgNPs) was evaluated by UV-Vis spectroscopy, while their hydrodynamic diameter and zeta potential were investigated by dynamic light spectroscopy (DLS). Transmission electron microscopy (TEM) analysis pointed out polydisperse NPs with quasi-spherical shapes. The newly synthesized nanoparticles showed good antioxidant activity against the tested free radicals, DPPH, ABTS, and FRAP, the best results being obtained in the case of ABTS. BB-AgNPs exhibited good antibacterial activity on the tested Gram-positive and Gram-negative bacterial strains: herein , , , , , , and on yeast , respectively. The inhibition diameters varied between 7.67 ± 0.59 and 22.21 ± 1.06 mm, while the values obtained for minimum inhibitory concentration varied between 0.39 and 6.25 µg/mL. In vitro antiproliferative activity was tested on colon adenocarcinoma, ATCC HTB-37 cell line, and the results have shown that the green synthetized BB-AgNPs induced a substantial decrease in tumor cell viability in a dose-dependent manner with an IC50 ranging from 24.58 to 67.91 µg/mL. Consequently, more investigation is required to comprehend the processes of the cytotoxicity of AgNPs and develop strategies to mitigate their potentially harmful effects while harnessing their antimicrobial properties.
蜂粮（BB）是一种由蜂花粉制成的发酵混合物，富含蛋白质、氨基酸、脂肪酸、多酚、黄酮类化合物以及其他生物活性化合物，被认为是对人类有益的功能性食品。在本研究中，我们探索了一种创新的绿色合成胶体银纳米颗粒的方法，使用蜂粮提取物作为还原剂和稳定剂。对蜂粮提取物进行了初步化学表征。通过紫外-可见光谱法评估合成的银纳米颗粒（BB-AgNPs）的等离子体响应，同时通过动态光散射光谱法（DLS）研究其流体动力学直径和zeta电位。透射电子显微镜（TEM）分析指出纳米颗粒具有多分散性且形状近似球形。新合成的纳米颗粒对测试的自由基DPPH、ABTS和FRAP表现出良好的抗氧化活性，在ABTS测试中获得了最佳结果。BB-AgNPs对测试的革兰氏阳性和革兰氏阴性细菌菌株表现出良好的抗菌活性：在此分别为 、 、 、 、 、 ，对酵母 也有抗菌活性。抑菌直径在7.67±0.59至22.21±1.06毫米之间变化，而最小抑菌浓度的值在0.39至6.25微克/毫升之间变化。在结肠腺癌ATCC HTB-37细胞系上测试了体外抗增殖活性，结果表明绿色合成的BB-AgNPs以剂量依赖方式导致肿瘤细胞活力大幅下降，IC50范围为24.58至67.91微克/毫升。因此，需要更多研究来理解银纳米颗粒的细胞毒性过程，并制定策略来减轻其潜在有害影响，同时利用其抗菌特性。
