基于压电效应的食品中氯霉素检测免疫传感器

A piezoelectric immunosensor for chloramphenicol detection in food.

机构信息

Lipetsk State Technical University, 30 Moskovskaya St., Lipetsk 398600, Russian Federation. karaseva

出版信息

Talanta. 2012 May 15;93:44-8. doi: 10.1016/j.talanta.2011.12.047. Epub 2012 Jan 26.

DOI:10.1016/j.talanta.2011.12.047

Abstract

A piezoelectric immunosensor on the basis of electrogenerated polymer for detecting trace quantities of chloramphenicol, a drug which is used in veterinary for treatment and prevention of infectious diseases, was developed. The detection was conducted in a competitive format with the use of hapten-protein conjugate and monoclonal antibodies. The limit of detection is 0.2 ng ml(-1), the calibration curve is linear in the range of concentrations 0.5-100.0 ng ml(-1). Chloramphenicol was detected in food (meat, milk, egg, honey).

摘要

基于电生成聚合物的压电免疫传感器用于检测痕量氯霉素，氯霉素是一种用于兽医治疗和预防传染病的药物。检测采用半抗原-蛋白质缀合物和单克隆抗体进行竞争格式。检测限为 0.2ng/ml，校准曲线在 0.5-100.0ng/ml 浓度范围内呈线性。在食品（肉、奶、蛋、蜂蜜）中检测到了氯霉素。