基于 PVA-co-PE 纳米纤维膜的超灵敏无标记电化学免疫传感器用于牛奶中氯霉素残留的检测。
Ultrasensitive label-free electrochemical immunosensor based on PVA-co-PE nanofibrous membrane for the detection of chloramphenicol residues in milk.
机构信息
Fiber and Polymer Science, University of California, Davis, CA 95616, USA; Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, Egypt.
Fiber and Polymer Science, University of California, Davis, CA 95616, USA.
出版信息
Biosens Bioelectron. 2018 Oct 15;117:838-844. doi: 10.1016/j.bios.2018.07.025. Epub 2018 Jul 21.
An ultrasensitive label-free amperometric immunosensor for the detection of chloramphenicol (CAP) residues in milk has been developed by using a screen-printed carbon electrode laminated with a layer of poly (vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibrous membrane that is covalently immobilized with a CAP antibody (anti-CAP). The performance of the PVA-co-PE nanofiber membrane (PVA-co-PE NFM) on the electrode was compared with a PVA-co-PE casted membrane (PVA-co-PE CM), necessary fabrication steps and performance of the sensors were investigated by electrochemical impedance spectroscopy (EIS). The application of the PVA-co-PE NFM decreased the electron-transfer-resistance by about 4 times compared with a conventional PVA-co-PE casted membrane. Under the optimal conditions, the established immunosensor exhibited high sensitivity for determination of CAP in a range 0.01-10 ng mL, with a limit of detection of 0.0047 ng mL. In addition to the good selectivity, reusability and stability over time, the prepared immunosensor was successfully used in the detection of CAP in milk samples without any pretreatment.
一种超灵敏的无标记安培免疫传感器，用于检测牛奶中的氯霉素（CAP）残留，该传感器是通过使用印刷碳电极层压一层聚（乙烯醇-co-乙烯）（PVA-co-PE）纳米纤维膜来制备的，该膜通过共价固定氯霉素抗体（抗-CAP）。将 PVA-co-PE 纳米纤维膜（PVA-co-PE NFM）在电极上的性能与 PVA-co-PE 铸造膜（PVA-co-PE CM）进行了比较，通过电化学阻抗谱（EIS）研究了传感器的必要制备步骤和性能。与传统的 PVA-co-PE 铸造膜相比，PVA-co-PE NFM 的电子转移电阻降低了约 4 倍。在最佳条件下，建立的免疫传感器对 CAP 的检测范围为 0.01-10ngmL，检测限为 0.0047ngmL。除了具有良好的选择性、可重复使用性和长期稳定性外，该制备的免疫传感器还成功地用于未经任何预处理的牛奶样品中 CAP 的检测。
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