基于荧光的分析方法检测马兜铃酸衍生的 DNA 加合物。
A fluorescence-based analysis of aristolochic acid-derived DNA adducts.
机构信息
Department of Pharmacology, State University of New York at Stony Brook, Stony Brook, NY 11777, USA.
出版信息
Anal Biochem. 2012 Aug 1;427(1):49-51. doi: 10.1016/j.ab.2012.03.027. Epub 2012 Apr 4.
Abstract
Aristolochic acids (AAs), major components of plant extracts from Aristolochia species, form (after metabolic activation) pro-mutagenic DNA adducts in renal tissue. The DNA adducts can be used as biomarkers for studies of AA toxicity. Identification of these adducts is a complicated and time-consuming procedure. We present here a fast, nonisotopic, fluorescence-based assay for the detection of AA-DNA adducts in multiple samples. This approach allows analysis of AA adducts in synthetic DNA with known nucleotide composition and analysis of DNA adducts formed from chemically diverse AAs in vitro. The method can be applied to compare AA-DNA adduct formation in cells and tissues.
摘要
马兜铃酸（AAs）是马兜铃属植物提取物的主要成分，在肾组织中形成（经代谢激活后）促突变 DNA 加合物。这些 DNA 加合物可作为 AA 毒性研究的生物标志物。这些加合物的鉴定是一个复杂且耗时的过程。我们在此介绍一种快速、非同位素、基于荧光的检测方法，可用于检测多个样本中的 AA-DNA 加合物。该方法可用于分析具有已知核苷酸组成的合成 DNA 中的 AA 加合物，并分析体外化学多样性的 AA 形成的 DNA 加合物。该方法可用于比较细胞和组织中 AA-DNA 加合物的形成。
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