Laboratoire d'Ingénierie des Biomolécules, Nancy-Université, 2 avenue de Forêt de Haye, 54505 Vandoeuvre-Lès-Nancy, France.
Colloids Surf B Biointerfaces. 2012 Jun 15;95:75-81. doi: 10.1016/j.colsurfb.2012.02.024. Epub 2012 Mar 10.
Health benefits of unsaturated fatty acids have been demonstrated over the last decades. Nanotechnology provided new process to produce particles such as liposomes and nanoliposomes made of pure phospholipids. These techniques are already used in pharmaceutics to augment the bioavailability and the bioefficiency of drugs. The aim of this paper is to characterize and evaluate the potential of nanoliposomes made of three lecithins (soya, rapeseed and salmon) on cell culture in order to use them in the future as drug delivery systems for tissue engineering. We began to measure, with zetasizer, the radius size of liposomes particles which are 125.5, 136.7 and 130.3 nm respectively for rapeseed, soya and salmon lecithin. Simultaneously, solutions observed by TEM demonstrated the particles were made much of liposomes than droplet (emulsion). Finally, we found that the solutions of lecithins were enough stable over 5 days at 37 °C to be used in culture medium. We investigated the effect of soya, rapeseed and salmon lecithin liposome from 2mg/mL to 5.2 μg/mL on metabolic activity and cell proliferation on rat bone marrow stem cells (rBMSC) during 14 days. The results showed that the three lecithins (soya, rapeseed and salmon) improve cell proliferation at different concentration.
不饱和脂肪酸的健康益处在过去几十年中得到了证实。纳米技术提供了生产颗粒的新工艺，如由纯磷脂制成的脂质体和纳米脂质体。这些技术已经在药剂学中用于提高药物的生物利用度和生物效率。本文的目的是对由三种卵磷脂（大豆、油菜籽和三文鱼）制成的纳米脂质体进行表征和评估，以便将来将其用作组织工程的药物传递系统。我们开始用 Zetasizer 测量脂质体颗粒的半径大小，油菜籽、大豆和三文鱼卵磷脂的脂质体颗粒分别为 125.5、136.7 和 130.3nm。同时，TEM 观察到的溶液表明，这些颗粒主要是由脂质体而不是液滴（乳液）组成。最后，我们发现这些溶液在 37°C 下 5 天内足够稳定，可以用于培养基中。我们研究了 2mg/mL 至 5.2μg/mL 的大豆、油菜籽和三文鱼卵磷脂脂质体对大鼠骨髓间充质干细胞（rBMSC）代谢活性和细胞增殖的影响，持续 14 天。结果表明，三种卵磷脂（大豆、油菜籽和三文鱼）在不同浓度下都能提高细胞增殖。
