Liu Wei, Jiao Tiejun, Su Yuran, Wei Ran, Wang Zheng, Liu Jiacheng, Fu Na, Sui Lei
Department of Prosthodontics, School & Hospital of Stomatology, Tianjin Medical University Tianjin 30070 China
Department of Implant, School & Hospital of Stomatology, Tianjin Medical University Tianjin 30070 China
RSC Adv. 2022 Apr 19;12(19):11913-11922. doi: 10.1039/d2ra01398c. eCollection 2022 Apr 13.
Bone tissue engineering has emerged as a promising restorative strategy for bone reconstruction and bone defect repair. It is challenging to establish an appropriate scaffold with an excellent porous microstructure for bone defects and thereby promote bone repair. In this study, electrospinning as a simple and efficient technology was employed to fabricate a porous poly(3-hydroxybutyrate--4-hydroxybutyrate) (P34HB) scaffold coated with lecithin. The morphology, phase composition, and physical properties of the electrospun P34HB/lec scaffold were characterized. Meanwhile, cellular behaviors of bone marrow mesenchymal stem cells (BMSCs), including proliferation, adhesion, migration, osteogenic differentiation, and related gene expression, were also investigated. Finally, a rat subcutaneous implant model and a calvarial defect model were used to evaluated the biocompatibility and effect of these scaffolds on bone repair, respectively. The results demonstrated that these electrospun fibers were interwoven with each other to form the porous P34HB/lec scaffold and the addition of lecithin improved the hydrophilicity of the pure P34HB scaffold, enhanced the efficiency of cell migration, and decreased inflammatory response. Furthermore, the results showed that P34HB/lec scaffold had excellent biocompatibility, improved the vascularization, and promoted the bone regeneration. All these results indicated that nanofibers of P34HB scaffolds in combination with the lecithin could exert a synergistic effect on promoting osteogenesis and regeneration of bone defects; thus, the P34HB scaffold with lecithin showed great application potential for bone tissue engineering.
骨组织工程已成为一种用于骨重建和骨缺损修复的有前景的修复策略。为骨缺损建立具有优异多孔微结构的合适支架并由此促进骨修复具有挑战性。在本研究中，采用静电纺丝这种简单高效的技术制备了一种涂有卵磷脂的多孔聚（3-羟基丁酸酯-4-羟基丁酸酯）（P34HB）支架。对静电纺丝的P34HB/lec支架的形态、相组成和物理性能进行了表征。同时，还研究了骨髓间充质干细胞（BMSCs）的细胞行为，包括增殖、黏附、迁移、成骨分化及相关基因表达。最后，分别使用大鼠皮下植入模型和颅骨缺损模型评估这些支架的生物相容性以及对骨修复的作用。结果表明，这些静电纺丝纤维相互交织形成多孔的P34HB/lec支架，卵磷脂的添加改善了纯P34HB支架的亲水性，提高了细胞迁移效率，并降低了炎症反应。此外，结果显示P34HB/lec支架具有优异的生物相容性，改善了血管生成，并促进了骨再生。所有这些结果表明，P34HB支架的纳米纤维与卵磷脂结合可在促进骨缺损的成骨和再生方面发挥协同作用；因此，含卵磷脂的P34HB支架在骨组织工程中显示出巨大的应用潜力。
