Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
J Mol Cell Cardiol. 2012 Jun;52(6):1203-12. doi: 10.1016/j.yjmcc.2012.03.012. Epub 2012 Mar 30.
Human cardiomyocytes derived from pluripotent stem cells hold great promise for cardiac cell therapy, disease modeling, drug discovery, and the study of developmental biology. Reaching these potentials fully requires the development of methods that enable efficient and robust generation of cardiomyocytes with expected characteristics. This review summarizes and discusses up-to-date methods that have been used to derive and enrich human cardiomyocytes from pluripotent stem cells, provides a brief overview of in vitro and in vivo characterization of these cardiomyocytes, and considers future advancement needed to further harness the power of these cells.
人多能干细胞诱导分化而来的心肌细胞在心脏细胞治疗、疾病建模、药物研发和发育生物学研究等领域具有广阔的应用前景。为了充分发挥这些潜能，需要开发出能够高效且稳定地获得具有预期特性的心肌细胞的方法。本文总结并讨论了目前用于从多能干细胞中获得和富集人心肌细胞的方法，简要概述了这些心肌细胞的体外和体内鉴定，并探讨了进一步利用这些细胞的功能所需的未来进展。
