Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS) & Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China.
Exp Cell Res. 2013 Jan 15;319(2):120-5. doi: 10.1016/j.yexcr.2012.09.011. Epub 2012 Sep 25.
Transplantation of embryonic stem cell- or induced pluripotent stem cell-derived cardiomyocytes (CMs) represents one promising approach for the treatment of myocardial infarction and failing hearts. Cardiac differentiation systems from these pluripotent stem cells (PSCs) can also be employed to better understand early developmental biology, drug discovery, toxicology testing, and disease modeling. A prerequisite to attain these goals is the ability to generate functional CMs in an efficient and reliable way. The lack of CM maturation must also be overcome, and appropriate methods for introducing PSC-CMs into heart while maintaining cell viability must be optimized. The past few years have seen major advances both in the differentiation, characterization and application of these cells to biological systems. Here we review recent progress, especially those performed in China, in basic stem cell biology involving studies of cardiogenesis and CMs through PSC differentiation, approaches for chamber-specific CM differentiation, maturation processes involving regulation of intracellular Ca(2+) signals, and applications.
胚胎干细胞或诱导多能干细胞衍生的心肌细胞（CMs）移植代表了一种有前途的治疗心肌梗死和心力衰竭的方法。这些多能干细胞（PSCs）的心脏分化系统也可用于更好地理解早期发育生物学、药物发现、毒理学测试和疾病建模。实现这些目标的前提是能够以有效和可靠的方式产生功能性的 CMs。还必须克服 CM 成熟度的缺乏，并且必须优化将 PSC-CMs 引入心脏的适当方法，同时保持细胞活力。在过去的几年中，这些细胞在生物系统中的分化、表征和应用方面都取得了重大进展。在这里，我们回顾了最近的进展，特别是在中国进行的涉及通过 PSC 分化研究心脏发生和 CMs、室特异性 CM 分化方法、涉及细胞内 Ca(2+)信号调节的成熟过程以及应用的基础干细胞生物学研究方面的进展。
