Kim Yuri, Kim Jina, Lee Seung-Min, Lee Hyun Ah, Park Seolhyun, Kim Yesl, Kim Jung-Hyun
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Republic of Korea.
Biosci Biotechnol Biochem. 2012;76(4):737-44. doi: 10.1271/bbb.110857. Epub 2012 Apr 7.
The growing incidence of prostate cancer and the traditional use of Rubus coreanus Miquel (RCM) for prostate health led us to compare RCM extracts and to test their efficacy in inhibiting the growth of prostate cancer cells differing in androgen dependency. Ethanol extracts of unripe RCM (EUR) were more effective in reducing cell viability than water extracts or ripe RCM. EUR-induced growth inhibition, as indicated by significant reductions in numbers of proliferating cells and decreases in the protein levels of proliferating cell nuclear antigen (PCNA), cyclin D1 and CDK4, was greater in the androgen-dependent LNCaP cells than in the androgen-independent DU145 cells. EUR also induced mitochondrial-mediated apoptosis in prostate cancer cells by reducing Bcl-2 and Bcl-(X)L levels, but increased Bax levels. Nevertheless, the LNCaP cells were more sensitive to EUR-induced apoptosis and displayed sub-G1 and late apoptotic cell populations, whereas the DU145 cells did not. Our findings suggest that EUR suppresses the growth of prostate cancer cells by anti-proliferative and/or pro-apoptotic effects, and that these effects are stronger in androgen-dependent cells.
前列腺癌发病率的不断上升以及传统上悬钩子（Rubus coreanus Miquel，RCM）用于前列腺健康的情况，促使我们比较RCM提取物，并测试它们在抑制雄激素依赖性不同的前列腺癌细胞生长方面的功效。未成熟RCM的乙醇提取物（EUR）在降低细胞活力方面比水提取物或成熟RCM更有效。EUR诱导的生长抑制，表现为增殖细胞数量显著减少以及增殖细胞核抗原（PCNA）、细胞周期蛋白D1和细胞周期蛋白依赖性激酶4（CDK4）蛋白水平降低，在雄激素依赖性LNCaP细胞中比在雄激素非依赖性DU145细胞中更明显。EUR还通过降低Bcl-2和Bcl-XL水平，但增加Bax水平，诱导前列腺癌细胞中线粒体介导的凋亡。然而，LNCaP细胞对EUR诱导的凋亡更敏感，并显示出亚G1期和晚期凋亡细胞群，而DU145细胞则没有。我们的研究结果表明，EUR通过抗增殖和/或促凋亡作用抑制前列腺癌细胞的生长，并且这些作用在雄激素依赖性细胞中更强。
