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通过抑制 NF-κB 和 MAP 激酶来研究朝鲜悬钩子的抗炎作用。

Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP Kinase.

机构信息

Department of Home Economics Education, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea.

Functional Food & Nutrition Division, Rural Development Administration, Gyeonggi 441-853, Korea.

出版信息

Nutr Res Pract. 2014 Oct;8(5):501-8. doi: 10.4162/nrp.2014.8.5.501. Epub 2014 May 15.

DOI:10.4162/nrp.2014.8.5.501
PMID:25324928
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4198961/
Abstract

BACKGROUND/OBJECTIVES: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.

MATERIALS/METHODS: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.

RESULTS

UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.

CONCLUSIONS

In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.

摘要

背景/目的：悬钩子属植物（Rubus Coreanus Miquel）（RCM）作为一种传统的韩国药物，可减轻癌症和类风湿性关节炎等慢性炎症性疾病。然而，其机制尚未阐明。在这项研究中，我们使用 RAW 264.7 细胞检查了 RCM 的抗炎作用及其可能的机制。

材料/方法：制备未成熟 RCM 乙醇提取物（UE）、未成熟 RCM 水提取物（UH）、成熟 RCM 乙醇提取物（RE）和成熟 RCM 水提取物（RH）。用 LPS 处理诱导炎症反应，并评估促炎介质（iNOS、COX-2、TNF-α、IL-1β和 IL-6）和 NO 和 PGE2 的表达。为了确定 RCM 的抗炎机制，我们测量了 NF-κB 和 MAPK 活性。

结果

UE 和 UH 处理显著降低了 NF-κB 激活和 JNK 和 p38 磷酸化，并降低了转录活性降低 iNOS、COX-2 和促炎细胞因子的表达以及 NO 和 PGE2 的产生。RE 和 RH 处理通过抑制 JNK 和 p38 磷酸化来降低 IL-1β 和 IL-6 的表达。

结论

在这项研究中，我们表明 RCM 通过抑制促炎介质的表达具有抗炎作用。特别是，未成熟的 RCM 通过抑制 NF-κB 和 MAPK 激活显示出强大的抗炎作用。这些发现表明，未成熟的 RCM 可能被用作减轻慢性炎症反应的潜在功能材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/4bfde55ff45d/nrp-8-501-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/a0756818c05a/nrp-8-501-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/d69697635c72/nrp-8-501-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/5ab099ff3f08/nrp-8-501-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/d96d0058d6a8/nrp-8-501-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/4bfde55ff45d/nrp-8-501-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/a0756818c05a/nrp-8-501-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/d69697635c72/nrp-8-501-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/5ab099ff3f08/nrp-8-501-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/d96d0058d6a8/nrp-8-501-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb01/4198961/4bfde55ff45d/nrp-8-501-g005.jpg

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