Kang Seong-Il, Shin Hye-Sun, Kim Hyo-Min, Hong Youn-Suk, Yoon Seon-A, Kang Seung-Woo, Kim Jeong-Hwan, Ko Hee-Chul, Kim Se-Jae
Department of Biology, Jeju National University, Jejusi, Jeju, Republic of Korea.
Biosci Biotechnol Biochem. 2012;76(4):755-61. doi: 10.1271/bbb.110868. Epub 2012 Apr 7.
This study explores the anti-obesity properties of a Sasa quelpaertensis leaf extract (SQE) in high-fat diet (HFD)-induced obese C57BL/6 mice and mature 3T3-L1 adipocytes. SQE administration with HFD for 70 d significantly decreased the body weight gain, adipose tissue weight, and serum total cholesterol and triglyceride levels in comparison with the HFD group. SQE administration also reduced the serum levels of glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and lactate dehydrogenase, and the accumulation of lipid droplets in the liver, suggesting a protective effect against HFD-induced hepatic steatosis. SQE administration restored the HFD-induced decreases with phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in epididymal adipose tissue. SQE also induced AMPK phosphorylation in mature 3T3-L1 adipocytes. These results suggest that SQE exerted an anti-obesity effect on HFD-induced obese mice by activating AMPK in adipose tissue and reducing lipid droplet accumulation in the liver.
本研究探讨了阔叶箬竹叶片提取物（SQE）对高脂饮食（HFD）诱导的肥胖C57BL/6小鼠和成熟3T3-L1脂肪细胞的抗肥胖特性。与高脂饮食组相比，高脂饮食同时给予SQE 70天可显著降低体重增加、脂肪组织重量以及血清总胆固醇和甘油三酯水平。给予SQE还可降低血清谷氨酸草酰乙酸转氨酶、谷氨酸丙酮酸转氨酶和乳酸脱氢酶水平，以及肝脏中脂滴的积累，表明对高脂饮食诱导的肝脂肪变性具有保护作用。给予SQE可恢复高脂饮食诱导的附睾脂肪组织中AMP激活蛋白激酶（AMPK）和乙酰辅酶A羧化酶（ACC）磷酸化的降低。SQE还可诱导成熟3T3-L1脂肪细胞中AMPK磷酸化。这些结果表明，SQE通过激活脂肪组织中的AMPK和减少肝脏中脂滴的积累，对高脂饮食诱导的肥胖小鼠发挥抗肥胖作用。
