溶菌酶对白色念珠菌的损伤作用以及大鼠肺泡巨噬细胞与酵母样真菌接触后反应的变化。

Damaging effects of lyticase on Candida albicans and changes in the response of rat alveolar macrophages to the contact with yeast-like fungi.

作者信息

Morozov I A, Sachivkina N P, Kravtsov E G, Vasilyeva E A, Anokhina I V, Yashina N V, Dalin M V

机构信息

M. P. Chumakov Institute of Poliomyelitis and Viral Encephalites, Russian Academy of Medical Sciences, Moscow region, Russia.

出版信息

Bull Exp Biol Med. 2011 Oct;151(6):705-8. doi: 10.1007/s10517-011-1421-x.

DOI:10.1007/s10517-011-1421-x

PMID:22485213

Abstract

Changes in the cell wall of yeast-like fungi Candida albicans caused by bacterial enzyme lyticase were studied under an electron microscope. The results were compared with the findings on phagocytosis of lyticase-treated Candida albicans by rat alveolar macrophages. It was shown that destruction of the mannan and fibrous outer layers of the cell wall of yeast-like fungi treated with the enzyme led to the release of intracellular organelles and their content. Lyticase treatment reduced the resistance of Candida albicans to intracellular digestion by macrophages contributing to completion of phagocytosis.

摘要

在电子显微镜下研究了细菌酶溶菌酶引起的酵母样真菌白色念珠菌细胞壁的变化。将结果与大鼠肺泡巨噬细胞对经溶菌酶处理的白色念珠菌的吞噬作用研究结果进行了比较。结果表明，用该酶处理的酵母样真菌细胞壁的甘露聚糖和纤维外层被破坏，导致细胞内细胞器及其内容物释放。溶菌酶处理降低了白色念珠菌对巨噬细胞细胞内消化的抵抗力，有助于吞噬作用的完成。