Lindbo J A, Dougherty W G
Department of Microbiology, Oregon State University, Corvallis 97331-3804.
Virology. 1992 Aug;189(2):725-33. doi: 10.1016/0042-6822(92)90595-g.
Transgenic tobacco plants which express untranslatable sense or antisense forms of the tobacco etch virus potyvirus (TEV) coat protein (CP) gene sequence have been generated. One of seven transgenic plant lines expressing a CP gene antisense transcript showed an attenuation of symptoms when inoculated with TEV. Three of ten transgenic plant lines expressing untranslatable sense transcripts did not develop symptoms when inoculated with TEV. These lines were resistant to either aphid or mechanically transmitted TEV. In contrast to CP-mediated resistance reported for other viruses, resistance was (1) mediated by an RNA molecule; (2) TEV-specific (i.e., "broad-spectrum resistance" was not observed); (3) independent of inoculum levels; (4) not dependent on plant size and; (5) due to decreased levels of virus replication. Protoplast experiments were used to demonstrate that resistant plant lines did not support the production of virus protein and progeny virus at wild-type levels.
已经培育出了表达烟草蚀纹病毒马铃薯Y病毒（TEV）外壳蛋白（CP）基因序列不可翻译的正义或反义形式的转基因烟草植株。在七个表达CP基因反义转录本的转基因植株系中，有一个在接种TEV时症状减轻。在十个表达不可翻译正义转录本的转基因植株系中，有三个在接种TEV时未出现症状。这些株系对蚜虫传播或机械传播的TEV具有抗性。与报道的其他病毒的CP介导抗性不同，这种抗性（1）由RNA分子介导；（2）具有TEV特异性（即未观察到“广谱抗性”）；（3）与接种量无关；（4）不依赖于植株大小；（5）是由于病毒复制水平降低。原生质体实验用于证明抗性植株系不能支持野生型水平的病毒蛋白和子代病毒的产生。
