Shyh-Dar Li, Song Li, Leaf Huang
Division of Molecular Pharmaceutics, School of Pharmacy, University of North Carolina at Chapel Hill, North Carolina 27599, USA.
CSH Protoc. 2006 Jun 1;2006(1):pdb.prot4448. doi: 10.1101/pdb.prot4448.
INTRODUCTION Lipoplex (cationic liposome-DNA complex) is formed via electrostatic interaction of anionic nucleic acids with cationic liposomes. A thin film of lipids is dried on the bottom of a glass tube and rehydrated in an aqueous solution. The resulting liposome suspension is passed through polycarbonate filters of desired pore size. This protocol also describes the preparation, physical properties, and biological activity of liposome-polycation-DNA (LPD) nanoparticles. The LPD nanoparticles contain a highly condensed DNA core surrounded by lipid bilayers with an average size of ~100 nm. The nanoparticle complex is injected into mice, and expression of the transfected DNA is monitored with an appropriate assay.
引言 脂质体复合物（阳离子脂质体 - DNA 复合物）是通过阴离子核酸与阳离子脂质体的静电相互作用形成的。脂质薄膜在玻璃管底部干燥，然后在水溶液中复水合。所得脂质体悬浮液通过所需孔径的聚碳酸酯滤膜。本方案还描述了脂质体 - 聚阳离子 - DNA（LPD）纳米颗粒的制备、物理性质和生物学活性。LPD 纳米颗粒包含一个被脂质双层包围的高度浓缩的 DNA 核心，平均尺寸约为 100 nm。将纳米颗粒复合物注射到小鼠体内，并用适当的检测方法监测转染 DNA 的表达。
