• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Purification of nucleic acids by extraction with phenol:chloroform.

作者信息

Sambrook Joseph, Russell David W

出版信息

CSH Protoc. 2006 Jun 1;2006(1):pdb.prot4455. doi: 10.1101/pdb.prot4455.

DOI:10.1101/pdb.prot4455
PMID:22485786
Abstract
摘要

相似文献

1
Purification of nucleic acids by extraction with phenol:chloroform.通过苯酚：氯仿萃取法纯化核酸。
CSH Protoc. 2006 Jun 1;2006(1):pdb.prot4455. doi: 10.1101/pdb.prot4455.
2
Determination of a universal nucleic acid extraction procedure for PCR detection of gastroenteritis viruses in faecal specimens.用于粪便标本中胃肠炎病毒PCR检测的通用核酸提取方法的确定。
J Virol Methods. 2002 Feb;100(1-2):1-16. doi: 10.1016/s0166-0934(01)00379-2.
3
Evaluation of three extraction methods for molecular detection of Schistosoma mansoni infection in human urine and serum samples.用于分子检测人尿液和血清样本中曼氏血吸虫感染的三种提取方法的评估
J Parasit Dis. 2015 Sep;39(3):499-507. doi: 10.1007/s12639-013-0385-3. Epub 2013 Nov 20.
4
Optimization of phenol-chloroform RNA extraction.苯酚-氯仿RNA提取的优化
MethodsX. 2018 May 30;5:599-608. doi: 10.1016/j.mex.2018.05.011. eCollection 2018.
5
Silicone lubricant enhances recovery of nucleic acids after phenol-chloroform extraction.硅胶润滑剂可提高酚-氯仿提取后核酸的回收率。
Nucleic Acids Res. 1993 Feb 11;21(3):781-2. doi: 10.1093/nar/21.3.781.
6
Differential recovery of mRNA transcripts using acid organic extraction techniques: quantification of abl mRNA abundance in the blowfly, Calliphora erythrocephala.使用酸性有机提取技术对mRNA转录本进行差异回收：红头丽蝇（Calliphora erythrocephala）中abl mRNA丰度的定量分析
SAAS Bull Biochem Biotechnol. 1993;6:21-30.
7
Isolation of High-Molecular-Weight DNA Using Organic Solvents.使用有机溶剂分离高分子量DNA
Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot093450. doi: 10.1101/pdb.prot093450.
8
Purification of RNA by SDS solubilization and phenol extraction.通过SDS溶解和苯酚提取纯化RNA。
Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5438. doi: 10.1101/pdb.prot5438.
9
[Comparing and evaluating six methods of extracting human genomic DNA from whole blood].[比较和评估从全血中提取人类基因组DNA的六种方法]
Fa Yi Xue Za Zhi. 2009 Apr;25(2):109-11, 114.
10
Efficiency evaluation of a DNA extraction and purification protocol on archival formalin-fixed and paraffin-embedded tissue.存档福尔马林固定石蜡包埋组织中 DNA 提取和纯化方案的效率评估。
Forensic Sci Int. 2010 Jan 30;194(1-3):e25-8. doi: 10.1016/j.forsciint.2009.09.004. Epub 2009 Sep 24.

引用本文的文献

1
DNA Methylation Status of Regulatory Regions of Apoptosis-Associated Genes in Dystropy «Huntington's Disease-Non-Small Cell Lung Cancer».营养不良（“亨廷顿舞蹈病 - 非小细胞肺癌”）中凋亡相关基因调控区域的DNA甲基化状态
Epigenomes. 2025 Aug 7;9(3):28. doi: 10.3390/epigenomes9030028.
2
GLP-1R associates with VAPB and SPHKAP at ERMCSs to regulate β-cell mitochondrial remodelling and function.胰高血糖素样肽-1受体（GLP-1R）在内质网-线粒体接触位点（ERMCSs）与VAPB和SPHKAP结合，以调节β细胞线粒体重塑和功能。
bioRxiv. 2025 Jul 20:2024.04.28.591531. doi: 10.1101/2024.04.28.591531.
3
The effects of Co-exposure of tobacco smoke with Dibenzo[a,l]pyrene diol epoxide on molecular targets and immune cells in the mouse oral cavity.
烟草烟雾与二苯并[a,l]芘二醇环氧化物共同暴露对小鼠口腔分子靶点和免疫细胞的影响。
Chem Biol Interact. 2025 Aug 5;420:111694. doi: 10.1016/j.cbi.2025.111694.
4
Unveiling the causative role of promoter variant rs113823671 in MMP7 in gallbladder cancer susceptibility.揭示启动子变体rs113823671在基质金属蛋白酶7（MMP7）中对胆囊癌易感性的致病作用。
Sci Rep. 2025 Jul 31;15(1):27958. doi: 10.1038/s41598-025-13833-3.
5
Metagenomic Analyses of Gut Bacteria of Two Sandfly Species from Western Ghats, India, Differing in Their Vector Competence for Leishmaniasis.对来自印度西高止山脉的两种白蛉肠道细菌的宏基因组分析，这两种白蛉在利什曼病的媒介能力上存在差异。
Microorganisms. 2025 Jul 9;13(7):1615. doi: 10.3390/microorganisms13071615.
6
Engineering of the Caspase-3 Gene in Recombinant CHO Cells Caused More Apoptosis Resistance and enhanced Recombinant Protein Production Than the BAX Gene.与BAX基因相比，重组CHO细胞中Caspase-3基因的工程改造导致了更强的抗凋亡能力和更高的重组蛋白产量。
Iran Biomed J. 2025 May 1;29(3):1-10. doi: 10.61186/ibj.4934.
7
Human CLOCK enhances neocortical function.人类生物钟蛋白增强新皮质功能。
Nat Neurosci. 2025 Jun 30. doi: 10.1038/s41593-025-01993-4.
8
Evidence That Dmrta2 Acts through Repression of in Cortical Patterning and Identification of a Mutation Impairing DNA Recognition Associated with Microcephaly in Human.Dmrta2通过抑制参与皮质模式形成的[相关基因]发挥作用的证据以及与人类小头畸形相关的损害DNA识别的突变鉴定。 （注：原文中“in Cortical Patterning and Identification of a Mutation Impairing DNA Recognition Associated with Microcephaly in Human.”部分似乎缺少某个关键基因名称，翻译时根据语境添加了“[相关基因]”以使句子更通顺，但具体需根据完整原文内容确定准确翻译。）
eNeuro. 2025 Jun 20;12(6). doi: 10.1523/ENEURO.0377-24.2025. Print 2025 Jun.
9
Bacteriophages as Vehicles for Antibiotic Resistance Genes in the Onyar River, Spain.西班牙奥尼亚尔河中作为抗生素抗性基因载体的噬菌体
Microb Ecol. 2025 May 8;88(1):41. doi: 10.1007/s00248-025-02541-0.
10
Species Composition, Ecological Preferences, and Chromosomal Polymorphism of Malaria Mosquitoes of the Crimean Peninsula and the Black Sea Coast of the Caucasus.克里米亚半岛及高加索黑海沿岸疟疾蚊子的种类组成、生态偏好和染色体多态性
Insects. 2025 Apr 1;16(4):367. doi: 10.3390/insects16040367.