硅胶润滑剂可提高酚-氯仿提取后核酸的回收率。 - Suppr

Suppr

超能文献

Silicone lubricant enhances recovery of nucleic acids after phenol-chloroform extraction.

作者信息

Mukhopadhyay T, Roth J A

机构信息

Department of Thoracic Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030.

出版信息

Nucleic Acids Res. 1993 Feb 11;21(3):781-2. doi: 10.1093/nar/21.3.781.

DOI:10.1093/nar/21.3.781

PMID:8441704

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309202/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b48/309202/e54960224289/nar00052-0407-a.jpg

相似文献

Silicone lubricant enhances recovery of nucleic acids after phenol-chloroform extraction.硅胶润滑剂可提高酚-氯仿提取后核酸的回收率。

Nucleic Acids Res. 1993 Feb 11;21(3):781-2. doi: 10.1093/nar/21.3.781.

Differential recovery of mRNA transcripts using acid organic extraction techniques: quantification of abl mRNA abundance in the blowfly, Calliphora erythrocephala.使用酸性有机提取技术对mRNA转录本进行差异回收：红头丽蝇（Calliphora erythrocephala）中abl mRNA丰度的定量分析

SAAS Bull Biochem Biotechnol. 1993;6:21-30.

Altered nucleic acid partitioning during phenol extraction or silica adsorption by guanidinium and potassium salts.胍盐和钾盐在苯酚抽提或硅胶吸附过程中对核酸分配的影响。

Anal Biochem. 2011 Dec 15;419(2):309-16. doi: 10.1016/j.ab.2011.08.047. Epub 2011 Aug 31.

Improved method for simultaneous isolation of proteins and nucleic acids.改进的蛋白质和核酸同时分离方法。

Anal Biochem. 2011 Apr 1;411(1):164-6. doi: 10.1016/j.ab.2010.11.020. Epub 2010 Nov 19.

The loss of ribosomal ribonucleic acid during the preparationof nucleic acid from certain plant tissues by the detergent-phenol method.在通过去污剂-苯酚法从某些植物组织制备核酸的过程中核糖体核糖核酸的损失。

Biochem J. 1968 Dec;110(3):605-6. doi: 10.1042/bj1100605.

SILICONE FLUID AS A LUBRICANT FOR ARTIFICIAL EYES.硅油作为人工眼的润滑剂

Am J Ophthalmol. 1964 Nov;58:767-74.

A phenol-chloroform protocol for extracting DNA from ancient samples.一种从古代样本中提取DNA的酚-氯仿方法。

Methods Mol Biol. 2012;840:13-9. doi: 10.1007/978-1-61779-516-9_2.

An assay for DNA fragmentation in apoptosis without phenol/chloroform extraction and ethanol precipitation.一种无需酚/氯仿提取和乙醇沉淀即可检测凋亡中DNA片段化的方法。

Anal Biochem. 1997 Mar 1;246(1):155-8. doi: 10.1006/abio.1997.2018.

Towards a universally adaptable method for quantitative extraction of high-purity nucleic acids from soil.迈向一种从土壤中定量提取高纯度核酸的通用适用方法。

J Microbiol Methods. 2008 Sep;75(1):19-24. doi: 10.1016/j.mimet.2008.04.009. Epub 2008 May 14.

An improved rapid method of isolating RNA from cultured cells by SDS-acid phenol/chloroform extraction.一种通过SDS-酸性苯酚/氯仿萃取从培养细胞中分离RNA的改良快速方法。

Biotechniques. 1994 Jan;16(1):56-7.

引用本文的文献

A Recurrent Nonsense Mutation in NECTIN4 Underlying Ectodermal Dysplasia-Syndactyly Syndrome with a Novel Phenotype in a Consanguineous Kashmiri Family.一个位于 NECTIN4 的新生无义突变导致的一种表型新颖的并指-外胚层发育不全综合征，该突变存在于一个亲缘关系较近的克什米尔家庭中。

Genet Res (Camb). 2023 Oct 4;2023:9999660. doi: 10.1155/2023/9999660. eCollection 2023.

A workflow for the relative quantification of multiple fish species from oceanic water samples using environmental DNA (eDNA) to support large-scale fishery surveys.利用环境 DNA（eDNA）对海洋水样中的多种鱼类进行相对定量的工作流程，以支持大规模渔业调查。

PLoS One. 2021 Sep 27;16(9):e0257773. doi: 10.1371/journal.pone.0257773. eCollection 2021.

Historical trends and new surveillance of Plasmodium falciparum drug resistance markers in Angola.安哥拉恶性疟原虫药物耐药性标志物的历史趋势和新监测。

Malar J. 2021 Apr 7;20(1):175. doi: 10.1186/s12936-021-03713-2.

Optimised biomolecular extraction for metagenomic analysis of microbial biofilms from high-mountain streams.用于高山溪流微生物生物膜宏基因组分析的优化生物分子提取方法

PeerJ. 2020 Oct 27;8:e9973. doi: 10.7717/peerj.9973. eCollection 2020.

Deposition of Iron in the Bone Marrow of a Murine Model of Hematopoietic Acute Radiation Syndrome.骨髓中铁的沉积：造血急性辐射综合征的鼠模型研究。

Exp Hematol. 2020 Apr;84:54-66. doi: 10.1016/j.exphem.2020.03.004. Epub 2020 Mar 30.

Paired RNA Radiocarbon and Sequencing Analyses Indicate the Importance of Autotrophy in a Shallow Alluvial Aquifer.成对的 RNA 放射性碳和测序分析表明自养在浅层冲积含水层中的重要性。

Sci Rep. 2019 Jul 17;9(1):10370. doi: 10.1038/s41598-019-46663-1.

Captopril mitigates splenomegaly and myelofibrosis in the Gata1 murine model of myelofibrosis.卡托普利减轻 Gata1 小鼠骨髓纤维化模型的脾肿大和骨髓纤维化。

J Cell Mol Med. 2018 Sep;22(9):4274-4282. doi: 10.1111/jcmm.13710. Epub 2018 Jul 4.

Sequence of the genome of the temperate, serotype-converting, Pseudomonas aeruginosa bacteriophage D3.温和型、血清型转换的铜绿假单胞菌噬菌体D3的基因组序列。

J Bacteriol. 2000 Nov;182(21):6066-74. doi: 10.1128/JB.182.21.6066-6074.2000.

本文引用的文献

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.采用酸性硫氰酸胍-苯酚-氯仿萃取法一步分离RNA的方法。

Anal Biochem. 1987 Apr;162(1):156-9. doi: 10.1006/abio.1987.9999.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验