固定化pH梯度制备型二维凝胶电泳：蛋白质的SDS-PAGE

Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: SDS-PAGE of Proteins.

作者信息

Stochaj Wayne R, Berkelman Tom, Laird Nancy

出版信息

CSH Protoc. 2006 Oct 1;2006(5):pdb.prot4559. doi: 10.1101/pdb.prot4559.

Abstract

INTRODUCTIONFollowing first-dimension IEF and equilibration of the IPG gel strips, the proteins are separated on the basis of their molecular weight in the second dimension on an SDS-PAGE gel. Systems for this separation are available from a variety of suppliers and are commonly found in many protein chemistry laboratories. This protocol describes a method for placement of the IPG strip and gives some recommended electrophoresis conditions for these second-dimension gels.

摘要

引言

在进行一维等电聚焦（IEF）以及IPG凝胶条平衡之后，蛋白质会依据其分子量在二维十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）凝胶上进行分离。用于这种分离的系统有众多供应商提供，并且在许多蛋白质化学实验室中普遍可见。本方案描述了一种IPG条带放置方法，并给出了这些二维凝胶的一些推荐电泳条件。