Stochaj Wayne R, Berkelman Tom, Laird Nancy
CSH Protoc. 2006 Oct 1;2006(5):pdb.prot4575. doi: 10.1101/pdb.prot4575.
INTRODUCTIONThis protocol describes a method for separating proteins based on their net charge using the technique of isoelectric focusing (IEF) on immobilized pH gradient (IPG) gels. This method serves as the first dimension of the 2D separation. The method described in this protocol utilizes a flatbed unit; however, self-contained instruments for IEF are also available.
引言
本方案描述了一种基于蛋白质净电荷，利用固定化pH梯度（IPG）凝胶上的等电聚焦（IEF）技术分离蛋白质的方法。该方法作为二维分离的第一维。本方案中描述的方法使用平板装置；不过，也有用于IEF的独立仪器。
