Stochaj Wayne R, Berkelman Tom, Laird Nancy
CSH Protoc. 2006 Oct 1;2006(5):pdb.prot4574. doi: 10.1101/pdb.prot4574.
INTRODUCTIONThis protocol describes a method for rehydration of IPG gel strips in preparation for their use for isoelectric focusing (IEF) on immobilized pH gradient (IPG) gels. Following rehydration, IEF can be performed using either a flatbed unit or a self-contained instrument.
引言
本方案描述了一种用于IPG凝胶条复水的方法,以准备在固定化pH梯度(IPG)凝胶上进行等电聚焦(IEF)。复水后,可使用平板装置或独立仪器进行IEF。