Department of Radiological Sciences, University of Texas Health Science Center, San Antonio, TX, USA.
Photochem Photobiol. 2012 Nov-Dec;88(6):1385-95. doi: 10.1111/j.1751-1097.2012.01156.x. Epub 2012 May 14.
Certain phytochemicals, such as the stilbene, resveratrol (RES, found in red grapes and berries), and the triterpenoid, ursolic acid (UA, found in waxy berries and herbs such as rosemary and oregano), have antioxidant, anti-inflammatory and antiproliferative effects. Two human-derived cell lines, hTERT-RPE with a nonmalignant phenotype derived from retinal pigment epithelium, and ATCC CRL-11147 derived from a malignant skin melanoma, were used as in vitro models of photooxidative stress produced by exposure to the broadband output of a 150 W Hg vapor arc lamp at an irradiance of 19-26 mW cm(-2). In untreated cells, UV-VIS broadband light exposure produced a loss of proliferative ability, an activation of NF-κB and an increase in protein carbonyl adducts at 24 h postexposure. Pretreatment of the cells with RES or UA at 1-2 μmsignificantly reduced the amount of phosphorylated NF-κB at 24 h postexposure. RES pretreatment reduced the burden of light-induced protein carbonyl adducts by up to 25% in exposed cells. UA treatment markedly increased the sensitivity of melanoma cells to UV radiation, while conferring some photoprotection to RPE cells. These observations indicate that phytochemicals modulate the cellular response to photochemical stress by interacting with specific cell-signaling pathways.
某些植物化学物质，如白藜芦醇（RES，存在于红葡萄和浆果中）和三萜酸熊果酸（UA，存在于蜡质浆果和香草如迷迭香和牛至中），具有抗氧化、抗炎和抗增殖作用。两种人源细胞系，hTERT-RPE 具有非恶性表型，源自视网膜色素上皮，和 ATCC CRL-11147 源自恶性皮肤黑色素瘤，被用作体外模型，用于模拟暴露于宽带输出的光氧化应激 150 W 汞蒸气弧灯在辐照度为 19-26 mW cm(-2)。在未经处理的细胞中，UV-VIS 宽带光暴露会在暴露后 24 小时内导致增殖能力丧失、NF-κB 激活和蛋白质羰基加合物增加。用 1-2 μM 的 RES 或 UA 预处理细胞可显著减少暴露后 24 小时时磷酸化 NF-κB 的数量。RES 预处理可使暴露细胞中的光诱导蛋白质羰基加合物负担减少多达 25%。UA 处理显著增加了黑色素瘤细胞对紫外线辐射的敏感性，同时赋予 RPE 细胞一定的光保护作用。这些观察结果表明，植物化学物质通过与特定的细胞信号通路相互作用来调节细胞对光化学应激的反应。
