STAFF Institute, Ippaizuka, Tsukuba, Ibaraki, Japan.
Anim Genet. 2012 Jun;43(3):348-51. doi: 10.1111/j.1365-2052.2011.02247.x. Epub 2011 Aug 15.
The chicken major histocompatibility complex (MHC-B locus) has a strong association with resistance and susceptibility to numerous diseases. We have found a B haplotype designated WLA that associated with the regression of tumours caused by Rous sarcoma virus J strain (RSV-J). Haplotype WLA was identical to the regressive B6 haplotype when partial genotyping was performed (Poultry Science, 89, 2010, 651). We then constructed a bacterial artificial chromosome (BAC) library from a WLA homozygote chicken to evaluate the structure of this regression haplotype and compared it to those of the B6 haplotype. Comparison between WLA and B6 above 59 kb within the 167 kb, including 14 genes from BG1 to BF2, revealed 75 SNPs and 14 indels. However, several genes were identical between WLA and B6, including the BF1 and BF2 genes, which encode a class I molecule previously suggested to be related to the regression phenotype. The BLB2 gene encoding the MHC class II beta chain showed the greatest diversity, with 19 non-synonymous SNPs. A comparison of WLA and B6 haplotpyes that are associated with tumour regression and RIRa and B24 haplotypes associated with tumour progression suggests that DMA1, DMA2, BRD2, TAPBP and BLB2 genes are not involved in the intensity of RSV J tumour regression.
鸡主要组织相容性复合体（MHC-B 基因座）与对多种疾病的抗性和易感性密切相关。我们发现了一个名为 WLA 的 B 单倍型，它与劳斯肉瘤病毒 J 株（RSV-J）引起的肿瘤消退有关。当进行部分基因分型时，WLA 单倍型与消退的 B6 单倍型相同（家禽科学，2010 年，89 卷，651 页）。然后，我们从 WLA 纯合鸡中构建了一个细菌人工染色体（BAC）文库，以评估这种消退单倍型的结构，并将其与 B6 单倍型进行比较。在 167 kb 内，包括从 BG1 到 BF2 的 14 个基因，WLA 与 B6 之间的比较显示了 75 个 SNP 和 14 个插入缺失。然而，WLA 和 B6 之间有几个基因是相同的，包括 BF1 和 BF2 基因，它们编码一种先前被认为与消退表型有关的 I 类分子。编码 MHC 类 II β链的 BLB2 基因显示出最大的多样性，有 19 个非同义 SNP。WLA 和 B6 单倍型与肿瘤消退有关，而 RIRa 和 B24 单倍型与肿瘤进展有关，这表明 DMA1、DMA2、BRD2、TAPBP 和 BLB2 基因不参与 RSV J 肿瘤消退的强度。
