STAFF Institute, Ippaizuka, Tsukuba, Ibaraki 305-0854, Japan.
Poult Sci. 2010 Apr;89(4):651-7. doi: 10.3382/ps.2009-00513.
The chicken MHC-B locus affects the response to several strains of Rous sarcoma virus (RSV). We evaluated the association between haplotypes of the MHC-B locus and responses to the J strain of RSV by using an F(2) experimental resource family constructed with tumor-regressive (White Leghorn) and tumor-progressive (Rhode Island Red) chickens. The MHC-B haplotypes were determined by genotyping of the microsatellite marker LEI0258 and MHC-B locus class I alpha chain 2 (BF2). Two haplotypes in the resource family, one associated with tumor regression and one with progression, were defined by these 2 markers. To discriminate more precisely the regressive haplotype in this family, we further developed 35 SNP markers at the MHC-B locus. Information on the haplotypes revealed here should be useful for identifying chickens with regression and progression phenotypes of J-strain RSV-induced tumors.
鸡 MHC-B 基因座影响对几种劳斯肉瘤病毒（RSV）株的反应。我们通过使用由肿瘤消退（白来航鸡）和肿瘤进展（罗得岛红鸡）鸡构建的 F2 实验资源家系，评估了 MHC-B 基因座单倍型与 J 株 RSV 反应之间的关联。通过微卫星标记 LEI0258 和 MHC-B 基因座 I 类 alpha 链 2（BF2）的基因分型确定 MHC-B 单倍型。这 2 个标记定义了资源家系中的 2 个单倍型，一个与肿瘤消退相关，一个与肿瘤进展相关。为了更精确地区分该家系中的消退单倍型，我们在 MHC-B 基因座进一步开发了 35 个 SNP 标记。此处揭示的有关单倍型的信息对于鉴定 J 株 RSV 诱导肿瘤的消退和进展表型的鸡将是有用的。
