Department of Civil and Environmental Engineering, Sejong University, Seoul, Republic of Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(7):1027-35. doi: 10.1080/10934529.2012.667320.
Air streams commonly emitted from industrial sources generally contain various mixtures of volatile organic compounds (VOCs), and these complex mixtures can present challenges with respect to bioreactor design and applications. In this study, therefore, a modified Monod-type model using interaction parameters was employed to describe the biodegradation kinetics of mixtures of aromatic compounds by a Pseudomonas isolate. In addition, the model and estimated parameters were utilized to predict the performance of a bubble-column bioreactor for the treatment of mixtures of benzene, toluene, p-xylene, and styrene (BTXS). Benzene, toluene and styrene, as individual substrates, were actively degraded by the bacterial culture, whereas p-xylene was not degraded as a single substrate. Relative to the single substrate experiments, the degradation of benzene and toluene was inhibited by the other compounds, while the degradation of styrene was significantly stimulated in the presence of the other BTXS compounds. The cometabolic degradation of p-xylene was observed in the presence of benzene and toluene. The estimated interaction parameters indicated that the degradation of benzene was substantially inhibited in the presence of styrene, whereas the degradation of styrene was strongly stimulated by toluene. The kinetic coefficients and interaction parameters were used to successfully predict the biodegradation kinetics and performance of a bioreactor subjected to the quaternary mixture. Overall, the model was able to provide reasonable predictions when substrate interactions, including inhibition, stimulation, and cometabolism, play significant roles in biodegradation processes.
空气流通常从工业来源排放，通常含有各种挥发性有机化合物 (VOC) 的混合物，这些复杂的混合物在生物反应器设计和应用方面带来了挑战。因此，在这项研究中，使用交互参数的修正 Monod 型模型被用于描述一种假单胞菌分离物对芳香族化合物混合物的生物降解动力学。此外，该模型和估计的参数被用于预测用于处理苯、甲苯、对二甲苯和苯乙烯 (BTXS) 混合物的鼓泡塔生物反应器的性能。作为单一底物，苯、甲苯和苯乙烯被细菌培养物积极降解，而对二甲苯则不能作为单一底物降解。与单一底物实验相比，苯和甲苯的降解被其他化合物抑制，而在存在其他 BTXS 化合物的情况下，苯乙烯的降解显著受到刺激。在苯和甲苯存在的情况下观察到对二甲苯的共代谢降解。估计的相互作用参数表明，苯乙烯的存在会显著抑制苯的降解，而甲苯会强烈刺激苯乙烯的降解。动力学系数和相互作用参数被用于成功预测生物反应器受到四元混合物的生物降解动力学和性能。总的来说，当基质相互作用（包括抑制、刺激和共代谢）在生物降解过程中起重要作用时，该模型能够提供合理的预测。
