Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
J Proteome Res. 2012 May 4;11(5):2890-903. doi: 10.1021/pr201266z. Epub 2012 Apr 17.
Porcine reproductive and respiratory syndrome (PRRS) has devastated the pig industry worldwide for almost 25 years, and its virus (PRRSV) preferentially infects and replicates in pulmonary alveolar macrophages (PAMs). To discover cellular protein responses in PRRSV-infected PAMs, two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the differentially expressed proteins between the PRRSV-infected groups and the controls. A total of 160 cellular proteins in PAMs that were significantly altered post-infection were identified. These differentially expressed proteins are related to the biological processes of virus binding, cell structure, signal transduction, cell adhesion, etc., and their interactions. This is the first report that analyzed the cellular protein profile of PRRSV-infected PAMs using iTRAQ technology, and this data provides important information to help understand the host response to PRRSV and to define the cellular requirements for the underlying mechanism of PRRSV replication and pathogenesis.
猪繁殖与呼吸综合征（PRRS）在全球范围内肆虐了近 25 年，其病毒（PRRSV）优先感染和复制肺泡巨噬细胞（PAMs）。为了发现 PRRSV 感染的 PAMs 中的细胞蛋白反应，采用二维液相色谱-串联质谱联用等离⼦体标记相对和绝对定量（iTRAQ）标记技术定量鉴定 PRRSV 感染组和对照组之间差异表达的蛋白质。共鉴定出 160 种在感染后明显改变的 PAMs 中的细胞蛋白质。这些差异表达的蛋白质与病毒结合、细胞结构、信号转导、细胞黏附等生物学过程及其相互作用有关。这是首次使用 iTRAQ 技术分析 PRRSV 感染的 PAMs 中的细胞蛋白谱的报告，该数据提供了重要信息，有助于了解宿主对 PRRSV 的反应，并确定 PRRSV 复制和发病机制的潜在机制的细胞要求。
