Shandong Key Lab of Animal Disease Control and Breeding, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
Sci Rep. 2018 Oct 9;8(1):15000. doi: 10.1038/s41598-018-32984-0.
Porcine reproductive and respiratory syndrome is an infectious disease that causes serious economic losses to the swine industry worldwide. To better understand the pathogenesis of the porcine reproductive and respiratory syndrome virus (PRRSV), three PRRSV strains with different molecular markers and virulence were used to infect MARC-145 cells. A total of 1804 proteins were identified, and 233 altered proteins and 72 signaling pathways involved in the proteomic profiling of virus-infected MARC-145 cells increased with the virulence of the PRRSV strain. The three types of viral strains shared a common pathway-the electron transport reaction in mitochondria-in the infected-MARC-145 cells. Moreover, the antisense pathway was the most variable of all significant signaling pathways for the highly virulent SX-1 strain, indicating that this unique pathway may be connected to the high virulence of the SX-1 strain. Our study is the first attempt to provide a proteome profile of MARC-145 cells infected with PRRSV strains with different virulence, and these findings will facilitate a deep understanding of the interactions between this virus and its host.
猪繁殖与呼吸综合征是一种传染病，它给全球的养猪业造成了严重的经济损失。为了更好地了解猪繁殖与呼吸综合征病毒（PRRSV）的发病机制，我们使用了三种具有不同分子标记和毒力的 PRRSV 株感染 MARC-145 细胞。共鉴定出 1804 种蛋白质，在病毒感染的 MARC-145 细胞的蛋白质组学分析中，233 种改变的蛋白质和 72 条信号通路与 PRRSV 株的毒力增加有关。这三种类型的病毒株在感染的 MARC-145 细胞中共享一条共同的途径——线粒体中的电子传递反应。此外，对于高毒力的 SX-1 株，反义途径是所有显著信号通路中最可变的，这表明这条独特的途径可能与 SX-1 株的高毒力有关。我们的研究首次尝试提供了不同毒力的 PRRSV 株感染 MARC-145 细胞的蛋白质组图谱，这些发现将有助于深入了解该病毒与其宿主之间的相互作用。
