Department of Oral Pathology, Antonio Pedro Hospital, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil.
J Oral Pathol Med. 2012 Sep;41(8):630-6. doi: 10.1111/j.1600-0714.2012.01126.x. Epub 2012 Jan 27.
Several cell types are associated with the development of cystic and tumoral odontogenic lesions. Among inflammatory cells, mast cells can be associated with their pathogenesis. The aim of this study was to analyze mast cells in periapical cysts, dentigerous cysts, and keratocystic odontogenic tumors.
Tissue sections were submitted to toluidine blue staining and immunohistochemistry with antibody anti-tryptase (clone G3). Mast cells were quantitated using Image-Pro Plus software to obtain the mean number of mast cells in three regions: epithelial, superficial portion of the fibrous wall and deep portion of the fibrous wall from 20 periapical cysts, 20 dentigerous cysts (six non-inflamed and 14 inflamed) and 20 keratocystic odontogenic tumors (four non-inflamed and 16 inflamed).
The mean number of mast cells detected per lesion by immunohistochemistry (4.1) was higher than by histochemistry (1.5) (P<0.0001). Inflamed dentigerous cysts and keratocystic odontogenic tumors showed a higher mean number of mast cells than non-inflamed lesions in all regions. The deep region from all cysts showed the highest mean number of degranulated mast cells, except for non-inflamed keratocystic odontogenic tumors analyzed by immunohistochemistry.
Immunohistochemical staining detected higher number of mast cells than histochemistry. The higher number of mast cells observed in inflamed lesions could indicate the participation of these cells in the inflammatory response in odontogenic lesions. The prevalence of degranulated mast cells in the deep region suggests intense activity of these cells, possibly related to growth of cystic lesions.
几种细胞类型与囊性和肿瘤性牙源性病变的发展有关。在炎症细胞中，肥大细胞可能与其发病机制有关。本研究旨在分析根尖囊肿、含牙囊肿和角化囊肿中的肥大细胞。
组织切片进行甲苯胺蓝染色和抗胰蛋白酶抗体（克隆 G3）免疫组织化学染色。使用 Image-Pro Plus 软件定量分析肥大细胞，以获得上皮、纤维壁浅层和纤维壁深层三个区域的肥大细胞平均值，从 20 个根尖囊肿、20 个含牙囊肿（6 个非炎症和 14 个炎症）和 20 个角化囊肿中获得（4 个非炎症和 16 个炎症）。
免疫组织化学法检测到的每个病变的平均肥大细胞数（4.1）高于组织化学法（1.5）（P<0.0001）。所有区域的炎症性含牙囊肿和角化囊肿的肥大细胞数均高于非炎症性病变。除非炎症性角化囊肿外，所有囊肿的深层区域显示出最高的脱颗粒肥大细胞平均数量，用免疫组织化学法分析。
免疫组织化学染色比组织化学法检测到更多的肥大细胞。在炎症性病变中观察到的肥大细胞数量增加可能表明这些细胞参与了牙源性病变的炎症反应。深层区域脱颗粒肥大细胞的发生率提示这些细胞的活性增强，可能与囊性病变的生长有关。
