Roi Ciprian, Negruțiu Meda Lavinia, Roi Alexandra, Riviș Mircea, Luca Ruxandra Elena, Raica Marius, Ceaușu Raluca Amalia, Motofelea Alexandru Cătălin, Gaje Pușa Nela
Department of Anesthesiology and Oral Surgery, Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, "Victor Babeș" University of Medicine and Pharmacy, Eftimie Murgu Sq. no. 2, 300041 Timisoara, Romania.
Department of Prostheses Technology and Dental Materials, Research Center in Dental Medicine Using Conventional and Alternative Technologies, Faculty of Dental Medicine, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania.
Biomedicines. 2023 Oct 5;11(10):2709. doi: 10.3390/biomedicines11102709.
Periapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the microvessel density (MVD), mast cell density (MCD) and heterogeneity of the encountered blood vessels. A total of 37 patients diagnosed with odontogenic periapical granulomas were included, and the gender distribution, age and localization of the pathological lesions was assessed. After the surgical removal of the periapical odontogenic granuloma, the collected tissue was fixed in 10% buffered formalin. Primary processing, morphological analysis and immunohistochemical staining was performed in order to characterize the altered tissue. The results outlined the presence of a high number of mast cells, especially in the area of the inflamed tissue; the high heterogeneity of the blood vessels; and increased MVD with positive CD34. The conclusions of the study focus on the key role of the mast cells and their implication in the initiation and development of the angiogenesis process, triggering the inflammatory response of the host. Nevertheless, periapical odontogenic granulomas develop as an inflammatory response to the interaction between the host's immune system and microbial invasion.
根尖牙源性肉芽肿是最常累及牙槽骨的病理情况之一，会导致诸如骨吸收、炎性浸润以及异常血管形成等严重后果。本研究旨在量化微血管密度（MVD）、肥大细胞密度（MCD）的存在情况以及所发现血管的异质性。共纳入37例诊断为牙源性根尖肉芽肿的患者，并评估了病理病变的性别分布、年龄及部位。手术切除根尖牙源性肉芽肿后，将采集的组织固定于10%中性福尔马林中。进行初步处理、形态学分析及免疫组织化学染色以对病变组织进行特征描述。结果显示存在大量肥大细胞，尤其是在炎症组织区域；血管高度异质性；以及CD34阳性的MVD增加。该研究的结论聚焦于肥大细胞的关键作用及其在血管生成过程起始和发展中的作用，引发宿主的炎症反应。然而，根尖牙源性肉芽肿是作为宿主免疫系统与微生物入侵之间相互作用的炎症反应而发展形成的。
