Biodiversity Institute of Ontario, Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada.
Mol Ecol. 2012 Apr;21(8):1794-805. doi: 10.1111/j.1365-294X.2012.05538.x.
Since 2005, advances in next-generation sequencing technologies have revolutionized biological science. The analysis of environmental DNA through the use of specific gene markers such as species-specific DNA barcodes has been a key application of next-generation sequencing technologies in ecological and environmental research. Access to parallel, massive amounts of sequencing data, as well as subsequent improvements in read length and throughput of different sequencing platforms, is leading to a better representation of sample diversity at a reasonable cost. New technologies are being developed rapidly and have the potential to dramatically accelerate ecological and environmental research. The fast pace of development and improvements in next-generation sequencing technologies can reflect on broader and more robust applications in environmental DNA research. Here, we review the advantages and limitations of current next-generation sequencing technologies in regard to their application for environmental DNA analysis.
自 2005 年以来，下一代测序技术的进步彻底改变了生物科学。通过使用特定的基因标记（如物种特异性 DNA 条码）来分析环境 DNA，已成为下一代测序技术在生态和环境研究中的一个关键应用。获取并行、大量的测序数据，以及不同测序平台的读取长度和通量的后续改进，正在以合理的成本实现对样本多样性的更好代表。新技术正在迅速发展，并有可能极大地加速生态和环境研究。下一代测序技术的快速发展和改进可以反映在环境 DNA 研究中更广泛和更强大的应用上。在这里，我们回顾了当前下一代测序技术在环境 DNA 分析中的应用的优势和局限性。
