European Directorate for the Quality of Medicines and Health Care, Strasbourg, France.
J Pharm Biomed Anal. 2012 Oct;69:50-63. doi: 10.1016/j.jpba.2012.03.019. Epub 2012 Mar 21.
Due to its wide field of application, sensitivity, wide range of linearity and the applicability to gradient elution, the most common detection technique for HPLC nowadays is UV/vis spectrophotometry. However, UV/vis detection comes to its limits when the analytes are lacking suitable chromophors or exhibit very different UV responses. In the past years, different types of evaporation/aerosol based HPLC detectors have been developed to fill this gap in HPLC detection. Amongst those, the corona-charged aerosol detector (CAD) is one of the most powerful and versatile representatives. In the recent past a variety of papers have been published, demonstrating the potential of the CAD in different fields of analytical chemistry. This paper is intended to provide an overview of the key performance characteristics and manifold applications for HPLC-CAD in the field of pharmaceutical analysis.
由于其广泛的应用领域、灵敏度、较宽的线性范围以及适用于梯度洗脱，目前 HPLC 最常用的检测技术是紫外/可见分光光度法。然而，当分析物缺乏合适的生色团或表现出非常不同的紫外响应时，紫外/可见检测就会达到其极限。在过去的几年中，已经开发出了不同类型的基于蒸发/气溶胶的 HPLC 检测器来填补 HPLC 检测中的这一空白。其中，荷电气溶胶检测器 (CAD) 是最强大和多功能的代表之一。在最近的一段时间里，已经发表了大量的论文，展示了 CAD 在分析化学不同领域的潜力。本文旨在概述 HPLC-CAD 在药物分析领域的关键性能特点和多种应用。
