University of Würzburg, Institute for Pharmacy and Food Chemistry, Am Hubland, 97074 Würzburg, Germany.
University of Würzburg, Institute for Pharmacy and Food Chemistry, Am Hubland, 97074 Würzburg, Germany.
J Chromatogr A. 2020 May 24;1619:460911. doi: 10.1016/j.chroma.2020.460911. Epub 2020 Jan 23.
High performance liquid chromatography (HPLC) methods with UV/vis detection are the most widespread analytical procedures in modern pharmaceutical applications, but reach their limitations when it comes to non-chromophore molecules. Hence, instead of using tiresome derivatization procedures, many liquid chromatography methods make use of the so-called aerosol-based universal detectors, namely the evaporative light scattering detector (ELSD), the condensation nucleation light scattering detector (CNLSD) and the charged aerosol detector (CAD). Amongst these, the CAD, being the youngest (introduced in 2005) of these three options, is often described as the most easy-to-use detector and is stated to exhibit sufficient sensitivity and good linearity of signal in a dedicated range of concentration. Therefore, this review sets its focus on the recent applications of the CAD for active pharmaceutical ingredients, excipient analysis as well as botanical applications. Alongside the post-column solvent addition techniques, the new CAD's ability to adjust the evaporation temperature and the possibility to use an integrated power function for signal linearization are reviewed as previously unavailable, new parameters for optimization.
高效液相色谱（HPLC）方法结合紫外/可见检测是现代制药应用中最广泛的分析程序，但在涉及非生色团分子时，它们会达到其局限性。因此，许多液相色谱方法没有使用繁琐的衍生化程序，而是利用所谓的基于气溶胶的通用检测器，即蒸发光散射检测器（ELSD）、冷凝核光散射检测器（CNLSD）和带电气溶胶检测器（CAD）。在这些检测器中，CAD 作为这三种选择中最新的（于 2005 年推出），通常被描述为最易于使用的检测器，并被认为在专门的浓度范围内具有足够的灵敏度和良好的信号线性度。因此，本综述将重点放在 CAD 在活性药物成分、赋形剂分析以及植物学应用中的最新应用上。除了柱后溶剂添加技术外，还回顾了 CAD 调整蒸发温度的新能力以及使用集成幂函数进行信号线性化的可能性，这些都是以前无法获得的新优化参数。
