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阳极液对鲶鱼片背景微生物群、沙门氏菌和单增李斯特菌的影响。

Effect of anolyte on background microflora, Salmonella, and Listeria monocytogenes on catfish fillets.

机构信息

Eastern Regional Research Center, U.S. Department of Agriculture, Agricultural Research Service, Wyndmoor, PA 19083-8551, USA.

出版信息

J Food Prot. 2012 Apr;75(4):765-70. doi: 10.4315/0362-028X.JFP-11-426.

DOI:10.4315/0362-028X.JFP-11-426
PMID:22488069
Abstract

Near-neutral electrolyzed water (anolyte), having a pH of 6.0 to 6.5 ± 0.02, oxidation reduction potential of greater than 700 mV, and a residual chlorine level of 10 to 200 ppm, was reported to have a potential use to decontaminate food surfaces. An electrolyzing cell was developed that is capable of producing neutral electrolyzed water containing a chlorine level of greater than 700 ppm in the form of hypochlorous acid (anolyte). Anolyte with a chlorine level of 300 ppm was used to determine its effect on Salmonella and Listeria monocytogenes cells after a 3-min contact. Transmission electron micrograph results showed disruption of the outer cellular membrane for both bacteria. The anolyte (300 ppm) was used as a washing solution to decontaminate catfish fillets inoculated with either Salmonella or L. monocytogenes. After a 3-min contact time with the anolyte, there was a 1-log reduction for Salmonella, and after 8 days of refrigerated storage (4°C), this bacterial reduction was maintained. There was no reduction of L. monocytogenes on the catfish fillet surfaces. The anolyte was an effective wash solution for Salmonella reduction on the catfish fillet surfaces.

摘要

近中性电解水(阳极液)的 pH 值为 6.0 至 6.5 ± 0.02,氧化还原电位大于 700 mV,余氯水平为 10 至 200 ppm,据报道具有消毒食品表面的潜力。开发了一种电解槽,能够以次氯酸(阳极液)的形式生产含氯水平大于 700 ppm 的中性电解水。使用含氯水平为 300 ppm 的阳极液来确定其对沙门氏菌和单核细胞增生李斯特菌细胞的影响,接触时间为 3 分钟。透射电子显微镜结果显示两种细菌的外细胞膜均受到破坏。将阳极液(300 ppm)用作洗涤溶液,以对感染沙门氏菌或单核细胞增生李斯特菌的鲶鱼片进行消毒。在与阳极液接触 3 分钟后,沙门氏菌的数量减少了 1 个对数级,在冷藏(4°C) 8 天后,这种细菌减少得到维持。鲶鱼片表面的单核细胞增生李斯特菌数量没有减少。阳极液是一种有效的鲶鱼片表面减少沙门氏菌的洗涤溶液。

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