Ibarra-Juarez L, Eisen L, Bolling B G, Beaty B J, Blitvich B J, Sanchez-Casas R M, Ayala-Sulca Y O, Fernandez-Salas I
Laboratorio de Entomología Médica, Universidad Autónoma de Nuevo León, San Nicolas de los Garza, Mexico.
Med Vet Entomol. 2012 Sep;26(3):351-4. doi: 10.1111/j.1365-2915.2012.01014.x. Epub 2012 Apr 10.
In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in 2006 and 2007. A total of 203 horses were serologically assayed for antibodies to WNV using an epitope-blocking enzyme-linked immunosorbent assay (bELISA). Seroprevalences for WNV in horses sampled in 2006 and 2007 were 26% and 45%, respectively. Mosquito collections in 2007 produced 7365 specimens representing 15 species. Culex mosquitoes were screened for WNV RNA and other genera (Mansonia, Anopheles, Aedes, Psorophora and Uranotaenia) were screened for flaviviruses using reverse-transcription (RT)-PCR. Two pools consisting of Culex spp. mosquitoes contained WNV RNA. Molecular species identification revealed that neither pool included Culex quinquefasciatus (Say) (Diptera:Culicidae) complex mosquitoes. No evidence of flaviviruses was found in the other mosquito genera examined. These data provide evidence that WNV is currently circulating in northern Mexico and that non-Cx. quinquefasciatus spp. mosquitoes may be participating in the WNV transmission cycle in this region.
在过去5年里，墨西哥北部仅报告过1例西尼罗河病毒（WNV）病的人类病例。为确定该病毒是否仍在该地区传播，2006年和2007年在墨西哥北部的新莱昂州开展了针对WNV的马匹和昆虫学监测。使用表位阻断酶联免疫吸附测定法（bELISA）对总共203匹马进行了WNV抗体的血清学检测。2006年和2007年采集的马匹中WNV的血清阳性率分别为26%和45%。2007年收集的蚊子标本有7365份，分属15个种类。对库蚊进行WNV RNA筛查，对其他属（曼蚊属、按蚊属、伊蚊属、骚蚊属和蓝带蚊属）使用逆转录（RT）-PCR进行黄病毒筛查。有两个库蚊属蚊子样本检测出含有WNV RNA。分子种类鉴定显示，两个样本中均不包括致倦库蚊（双翅目：蚊科）复合体蚊子。在所检测的其他蚊子属中未发现黄病毒感染迹象。这些数据证明，WNV目前正在墨西哥北部传播，且非致倦库蚊属蚊子可能参与了该地区的WNV传播循环。
