铜绿假单胞菌 CH34 菌株丙酮羧化酶的纯化与特性研究。
Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans strain CH34.
机构信息
Department of Proteomics and Microbiology, Interdisciplinary Center of Mass Spectrometry (CISMa), University of Mons, Mons, Belgium.
出版信息
Appl Environ Microbiol. 2012 Jun;78(12):4516-8. doi: 10.1128/AEM.07974-11. Epub 2012 Apr 6.
Abstract
Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteraceae family, genome analyses revealed that bacteria that possess an Acx, such as Cupriavidus metallidurans strain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex α(2)β(2)γ(2) and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.
摘要
丙酮羧化酶 (Acx) 是参与细菌降解丙酮的关键酶。除了螺旋菌科家族外，基因组分析表明，具有 Acx 的细菌，如金属硫杆菌 CH34 菌株，与土壤有关。CH34 的 Acx 形成异六聚体复合物 α(2)β(2)γ(2)，并且只能在 ATP 依赖性反应中将丙酮和 2-丁酮羧化为乙酰乙酸和 3-酮-2-甲基丁酸。
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