Department of Chemistry, School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, China.
J Biomed Mater Res A. 2012 Aug;100(8):1946-53. doi: 10.1002/jbm.a.34138. Epub 2012 Apr 10.
A novel, simple, and rapid method to fabricate thermoresponsive micropatterned substrate for cell adhesion, growth, and thermally induced detachment was developed. Thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAAm), was grafted onto the surface of polystyrene (PS) film with microstructure by plasma-induced graft polymerization technique. The thermoresponsive micropatterned films were characterized by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, hydrogen nuclear magnetic resonance ((1) H NMR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). These results indicated that the grafting ratio of PNIPAAm increased with increasing roughness of PS film. However, the microstructures on the substrate were not affected by grafted PNIPAAm. The optimal grafting conditions, such as plasma treatment time, monomer concentration, graft polymerization time, and graft medium were investigated. The thermoresponsive micropatterned films were investigated with the fibroblast cell (L929) adhesion, proliferation, and thermally induced detachment assay. The microstructure on the thermoresponsive micropatterned substrate facilitated cell adhesion above the lower critical solution temperature (LCST) of PNIPAAm and cell detachment below the LCST. Moreover, it can be used to regulate cell organization and tissue growth.
开发了一种新颖、简单、快速的方法来制造用于细胞粘附、生长和热诱导分离的温敏微图案化基底。通过等离子体引发接枝聚合技术，将温敏聚合物聚 N-异丙基丙烯酰胺（PNIPAAm）接枝到具有微结构的聚苯乙烯（PS）薄膜表面。通过衰减全反射傅里叶变换红外（ATR-FTIR）光谱、氢核磁共振（1H NMR）光谱、X 射线光电子能谱（XPS）和扫描电子显微镜（SEM）对温敏微图案化薄膜进行了表征。这些结果表明，随着 PS 薄膜粗糙度的增加，PNIPAAm 的接枝率增加。然而，基底上的微观结构不受接枝 PNIPAAm 的影响。研究了等离子体处理时间、单体浓度、接枝聚合时间和接枝介质等最佳接枝条件。用成纤维细胞（L929）粘附、增殖和热诱导分离试验研究了温敏微图案化薄膜。在低于 PNIPAAm 的低临界溶液温度（LCST）时，温敏微图案化基底上的微结构促进了细胞的粘附，而在 LCST 以下时则促进了细胞的分离。此外，它可用于调节细胞组织和组织生长。
