Viral Oncogenesis Group, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
Ark-Genomics, The Roslin Institute, R(D)SVS, University of Edinburgh, Division of Genetics and Genomics, Easter Bush, Midlothian EH25 9RG, UK.
J Gen Virol. 2012 Jul;93(Pt 7):1530-1536. doi: 10.1099/vir.0.040634-0. Epub 2012 Apr 4.
Duck enteritis virus (DEV) is an important herpesvirus pathogen associated with acute, highly contagious lethal disease in waterfowls. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast cultures, we identified several novel DEV-encoded micro (mi)RNAs. Unlike most mardivirus-encoded miRNAs, DEV-encoded miRNAs mapped mostly to the unique long region of the genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other, suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis.
鸭肠炎病毒（DEV）是一种重要的疱疹病毒病原体，与水禽的急性、高度传染性致死性疾病有关。我们通过对感染鸡胚成纤维细胞培养物的 RNA 进行深度测序，鉴定了几种新的 DEV 编码的 microRNAs。与大多数 mardivirus 编码的 miRNA 不同，DEV 编码的 miRNA 主要映射到基因组的独特长区域。DEV miR-D18 和 miR-D19 的前体相互重叠，这表明与 miRNA-offset RNA 相似，尽管只有 DEV-miR-D18-3p 在报告基因实验中具有功能。这些新 miRNA 的鉴定将增加不断增长的病毒编码 miRNA 列表，从而能够探索它们在发病机制中的作用。
