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筛选登革热病毒 2 型（DENV-2）感染白纹伊蚊（双翅目：蚊科）后差异表达的 microRNA 及其对 C6/36 细胞中 DENV-2 复制的影响。

Screening for differentially expressed miRNAs in Aedes albopictus (Diptera: Culicidae) exposed to DENV-2 and their effect on replication of DENV-2 in C6/36 cells.

机构信息

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, People's Republic of China.

Center for Disease Control and Prevention of Guangzhou Military Region, Guangzhou, 510507, People's Republic of China.

出版信息

Parasit Vectors. 2019 Jan 18;12(1):44. doi: 10.1186/s13071-018-3261-2.

DOI:10.1186/s13071-018-3261-2
PMID:30658692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6339288/
Abstract

BACKGROUND

The mosquito Aedes albopictus is an important vector for dengue virus (DENV) transmission. The midgut is the first barrier to mosquito infection by DENV, and this barrier is a critical factor affecting the vector competence of the mosquito. However, the molecular mechanism of the interaction between midgut and virus is unknown.

RESULTS

Six small libraries of Ae. albopictus midgut RNAs were constructed, three of which from mosquitoes that were infected with DENV-2 after feeding on infected blood, and another three that remained uninfected with DENV-2 after feeding on same batch of infected blood. A total of 46 differentially expressed miRNAs were identified of which 17 significant differentially expressed miRNAs were selected. Compared to microRNA expression profiles of mosquitoes that were uninfected with DENV-2, 15 microRNAs were upregulated and two were downregulated in mosquitoes that were infected with DENV-2. Among these differentially expressed microRNAs, miR-1767, miR-276-3p, miR-4448 and miR-622 were verified by stem-loop qRT-PCR in samples from seven-day-infected and uninfected midguts and chosen for an in vitro transient transfection assay. miR-1767 and miR-276-3p enhanced dengue virus replication in C6/36 cells, and miR-4448 reduced dengue virus replication.

CONCLUSIONS

To our knowledge, this study is the first to reveal differences in expression levels between mosquitoes infected and uninfected with DENV-2 after feeding on an infected blood meal. It provides useful information on microRNAs expressed in the midgut of Aedes albopictus after exposure to the virus.

摘要

背景

白纹伊蚊是登革病毒（DENV）传播的重要媒介。中肠是 DENV 感染蚊子的第一道屏障，该屏障是影响蚊子媒介能力的关键因素。然而，中肠与病毒相互作用的分子机制尚不清楚。

结果

构建了 6 个白纹伊蚊中肠 RNA 小文库，其中 3 个来自感染 DENV-2 后吸食感染血液的蚊子，另外 3 个来自吸食相同批次感染血液但未感染 DENV-2 的蚊子。共鉴定出 46 个差异表达的 miRNA，其中选择了 17 个差异表达显著的 miRNA。与未感染 DENV-2 的蚊子相比，感染 DENV-2 的蚊子中有 15 个 miRNA 上调，2 个 miRNA 下调。在这些差异表达的 miRNA 中，miR-1767、miR-276-3p、miR-4448 和 miR-622 在感染和未感染 7 天中肠的样本中通过茎环 qRT-PCR 验证，并选择用于体外瞬时转染试验。miR-1767 和 miR-276-3p 增强了 C6/36 细胞中的登革热病毒复制，而 miR-4448 降低了登革热病毒复制。

结论

据我们所知，这项研究首次揭示了感染和未感染 DENV-2 的蚊子在吸食感染血液后中肠表达水平的差异。它为暴露于病毒后白纹伊蚊中肠表达的 microRNAs 提供了有用的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/f99bd2c50139/13071_2018_3261_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/76269cdabd02/13071_2018_3261_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/64606dccefca/13071_2018_3261_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/0b1e3d6626a2/13071_2018_3261_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/6e96d355e680/13071_2018_3261_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/125e63bc51d8/13071_2018_3261_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/f99bd2c50139/13071_2018_3261_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/76269cdabd02/13071_2018_3261_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/64606dccefca/13071_2018_3261_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/0b1e3d6626a2/13071_2018_3261_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/6e96d355e680/13071_2018_3261_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/125e63bc51d8/13071_2018_3261_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11dd/6339288/f99bd2c50139/13071_2018_3261_Fig6_HTML.jpg

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Insects. 2016 Oct 30;7(4):58. doi: 10.3390/insects7040058.
3
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Parasit Vectors. 2024 Feb 17;17(1):69. doi: 10.1186/s13071-024-06161-4.
4
Aedes albopictus salivary adenosine deaminase is an immunomodulatory factor facilitating dengue virus replication.白纹伊蚊唾液腺腺苷脱氨酶是一种促进登革病毒复制的免疫调节因子。
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5
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6
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7
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5
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8
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9
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10
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