Zhu Chen-Chen, Gao Li, Zhao Zhong-Xiang, Lin Chao-Zhan
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Yao Xue Xue Bao. 2012 Jan;47(1):77-83.
A new triterpenoid saponin and fourteen known triterpenoids were isolated from the methanol extract of the stems and leaves of Callicarpa integerrima Champ, which is used in Chinese folk medicine for stopping bleeding, expelling the wind, dissipating stagnation, and treating scrofula, by using various chromatographies, such as silica gel, Sephadex LH-20 and RP-C18 column chromatography. Their structures were identified as a new compound 2alpha, 3beta, 19alpha, 23-tetrahydroxy-olean-12-en-28-oic acid-28-O-beta-D-glucopyranosyl-(1 --> 4)-beta-D-glucopyranoside (1), together with fourteen known compounds: oleanolic acid (2), 3-acetyl oleanolic acid (3), 3beta-O-acetyl ursolic acid (4), 2alpha-hydroxy-ursolic acid (5), 2alpha, 3beta, 19alpha, 23-tetrahydroxy-urs-12-en-28-oic acid (6), alpha-amyrin-3-O-beta-D-glucopyranoside (7), pomolic acid (8), betulinic acid (9), ursolic acid (10), 2alpha, 3beta, 19alpha, 23-tetrahydroxy-olean-12-en-28-oic acid (arjungenin) (11), 2alpha-hydroxy-oleanolic acid (12), hederagenin (13), 2alpha, 19alpha-dihydroxy-ursolic acid (14) and pruvuloside A (15), by the spectroscopic techniques of NMR, HMBC, IR and MS, separately. All these compounds were obtained from this plant for the first time, and compounds 3, 4 and 15 were isolated from genus Callicarpa L. for the first time.
从全缘叶紫珠（Callicarpa integerrima Champ）的茎和叶的甲醇提取物中，通过硅胶、Sephadex LH - 20和RP - C18柱色谱等多种色谱方法，分离得到了一种新的三萜皂苷和十四个已知的三萜类化合物。全缘叶紫珠在中国民间医学中用于止血、祛风、散瘀和治疗瘰疬。通过核磁共振（NMR）、异核多键相关谱（HMBC）、红外光谱（IR）和质谱（MS）等光谱技术，分别鉴定它们的结构为一种新化合物2α, 3β, 19α, 23 - 四羟基 - 齐墩果 - 12 - 烯 - 28 - 酸 - 28 - O - β - D - 吡喃葡萄糖基 - (1→4) - β - D - 吡喃葡萄糖苷（1），以及十四个已知化合物：齐墩果酸（2）、3 - 乙酰齐墩果酸（3）、3β - O - 乙酰熊果酸（4）、2α - 羟基熊果酸（5）、2α, 3β, 19α, 23 - 四羟基 - 乌苏 - 12 - 烯 - 28 - 酸（6）、α - 香树脂醇 - 3 - O - β - D - 吡喃葡萄糖苷（7）、坡模酸（8）、桦木酸（9）、熊果酸（10）、2α, 3β, 19α, 23 - 四羟基 - 齐墩果 - 12 - 烯 - 28 - 酸（阿尔琼皂苷元）（11）、2α - 羟基齐墩果酸（12）、常春藤皂苷元（13）、2α, 19α - 二羟基熊果酸（14）和普鲁武洛苷A（15）。所有这些化合物均首次从该植物中获得，化合物3、4和15首次从紫珠属（Callicarpa L.）中分离得到。
