Zhuang Yan-Shuang, Cai Hao, Liu Xiao, Cai Bao-Chang
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Yao Xue Xue Bao. 2012 Jan;47(1):84-7.
Reversed phase high performance liquid chromatography with diode array detector was employed for simultaneous determination of five main index components and specific chromatograms analysis in Xiaochaihu granules with a linear gradient elution of acetonitrile-water (containing 0.1% phosphoric acid) as mobile phase. The results showed that five main index components (baicalin, baicalein, wogonoside, wogonin, enoxolone) were separated well under the analytical condition. The linear ranges of five components were 0.518 - 16.576, 0.069 - 2.197, 0.167 - 5.333, 0.009 - 0.297 and 0.006 - 0.270 mg x g(-1), respectively. The correlation coefficients were 0.999 9, and the average recoveries ranged from 95% to 105%. Twelve common peaks were selected as the specific chromatograms of Xiaochaihu granules with baicalin as the reference peak. There were good similarities between the reference and the ten batches of samples. The similarity coefficients were no less than 0.9. The analytical method established is highly sensitive with strong specificity and it can be used efficiently in the quality control of Xiaochaihu granules.
采用反相高效液相色谱法结合二极管阵列检测器，以乙腈 - 水（含0.1%磷酸）为流动相进行线性梯度洗脱，同时测定小柴胡颗粒中的5种主要指标成分并进行特征图谱分析。结果表明，在该分析条件下5种主要指标成分（黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、甘草酸）分离良好。5种成分的线性范围分别为0.518 - 16.576、0.069 - 2.197、0.167 - 5.333、0.009 - 0.297和0.006 - 0.270 mg·g⁻¹，相关系数均为0.999 9，平均回收率在95%至105%之间。选取12个共有峰作为以黄芩苷为参照峰的小柴胡颗粒特征图谱，参照图谱与10批样品图谱相似度良好，相似度系数均不低于0.9。所建立的分析方法灵敏度高、专属性强，可有效用于小柴胡颗粒的质量控制。
