Liu Qingchun, Zhao Junning, Yan Liangchun, Yi Jinhai, Song Jun
Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.
Zhongguo Zhong Yao Za Zhi. 2010 Mar;35(6):708-10. doi: 10.4268/cjcmm20100610.
To establish a HPLC-PDA method for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang.
A Symmetry Shield RP18 (4.6 mm x 250 mm, 5.0 microm) was used with a mobile phase of acetonitrile-0.01% H3PO4 in gradient elution. The detection wavelength was 251 nm,the flow rate was 0.45 mL x min(-1) and the column temperature was maintained at 30 degrees C.
The accuracy, precision, sensitivity, specificity and linearity of this method met the requirements. The contents of the five effective fractions were determined simultaneously.
The method is rapid,simple and accurate and it can be suitable for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang simultaneously.
建立一种高效液相色谱-光电二极管阵列检测法（HPLC-PDA），用于测定小柴胡汤中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素和甘草酸的含量。
采用Symmetry Shield RP18（4.6 mm×250 mm，5.0 µm）色谱柱，以乙腈-0.01%磷酸为流动相进行梯度洗脱。检测波长为251 nm，流速为0.45 mL·min⁻¹，柱温保持在30℃。
该方法的准确度、精密度、灵敏度、特异性和线性均符合要求。可同时测定5种有效成分的含量。
该方法快速、简便、准确，适用于同时测定小柴胡汤中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素和甘草酸的含量。
