Lin S J, Tseng H H, Wen K C, Suen T T
National Laboratories of Foods and Drugs, Department of Health, Nankang, Taipei, Taiwan.
J Chromatogr A. 1996 Apr 12;730(1-2):17-23. doi: 10.1016/0021-9673(95)01270-2.
High-performance liquid chromatography was employed to determine the contents of several marker substances such as gentiopicroside, mangiferin, palmatine, berberine, baicalin, wogonin and glycyrrhizin in Sann-Joong-Kuey-Jian-Tang. The separation was performed on a Cosmosil 5C18-AR column by gradient elution with 0.03% (v/v) phosphoric acid-acetonitrile (0 min, 90:10; 10 min, 87:13; 17-27 min, 77:23; 40 min, 62:38; 50 min, 55:45) as the mobile phase at a flow-rate of 1.0 ml/min, with detection at 254 nm. n-Propylparaben was used as the internal standard and seven regression equations revealed linear relationships between the peak-area ratios (marker substances/internal standard) and concentrations. The repeatability and reproducibility (relative standard deviation) of the method were in the ranges 0.02-1.78% and 1.44-4.95%, respectively.
采用高效液相色谱法测定三黄苦参煎剂中龙胆苦苷、芒果苷、巴马汀、小檗碱、黄芩苷、汉黄芩素和甘草酸等几种标志性成分的含量。分离在Cosmosil 5C18-AR柱上进行，以0.03%（v/v）磷酸-乙腈（0分钟，90:10；10分钟，87:13；17 - 27分钟，77:23；40分钟，62:38；50分钟，55:45）为流动相进行梯度洗脱，流速为1.0 ml/min，检测波长为254 nm。对羟基苯甲酸丙酯用作内标，七个回归方程显示了峰面积比（标志性成分/内标）与浓度之间的线性关系。该方法的重复性和重现性（相对标准偏差）分别在0.02 - 1.78%和1.44 - 4.95%范围内。
