Yang Feng-Yun, Fu Ting-Ming, Guo Li-Wei, Liu Feng, Zhang Wei
Key Laboratory of Separation Engineering for Chinese Medicine Compound, Nanjing University of Chinese Medicine, Nanjing 210029, China.
Yao Xue Xue Bao. 2012 Jan;47(1):110-5.
This is to report the study of degradation of earthworm extracts prepared by wet superfine grinding in simulated gastrointestinal environment. Enzymatic reactions were terminated by adjusting the solution pH or using membrane bioreactor principle. Earthworm protein concentration change was detected by Bradford method, the degraded state of protein was described with SDS-PAGE technology, and the degraded state of small molecule substances was detected by HPLC. The results showed that earthworm protein degraded completely in artificial gastric juice. High molecular weight protein degraded greatly in artificial intestinal fluid, while low molecular weight protein was not significantly degraded. Small molecular substances degradation did not degrade in artificial gastric juice, while they degraded obviously in artificial intestinal fluid, there is even new small molecule substance appeared. Finally it is concluded that the substance that having therapeutic effects in vivo may be some degraded peptide, amino acid and stable small molecules existed in artificial intestinal fluid.
本文报道了在模拟胃肠道环境中，通过湿法超微粉碎制备的蚯蚓提取物的降解研究。通过调节溶液pH值或利用膜生物反应器原理终止酶促反应。采用考马斯亮蓝法检测蚯蚓蛋白质浓度变化，用SDS-PAGE技术描述蛋白质的降解状态，并用HPLC检测小分子物质的降解状态。结果表明，蚯蚓蛋白质在人工胃液中完全降解。高分子量蛋白质在人工肠液中大量降解，而低分子量蛋白质降解不明显。小分子物质在人工胃液中不降解，而在人工肠液中明显降解，甚至出现了新的小分子物质。最后得出结论，体内具有治疗作用的物质可能是人工肠液中存在的一些降解肽、氨基酸和稳定的小分子。
