Mei Lijun, Lin Chen, Lei Shanshan, Xu Li, Fan Yong-Sheng
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.
Evid Based Complement Alternat Med. 2018 Apr 29;2018:8265150. doi: 10.1155/2018/8265150. eCollection 2018.
in Chinese medicine has long been used as an effective treatment against rheumatoid arthritis (RA). The present research further investigated the effects of peptides extracted from the crude on the RA rat model. Extracted peptides were separated by parameter-optimized ion-exchange chromatography (IEC), peptide fractions were further analysed by MALDI-TOF/TOF MS, and nano-LC-MS/MS acquired mass spectra were further characterized using Mascot software, which ranks the best matches in the NCBI database. RT-PCR results in RAW264.7 cells indicated that peptide components had inhibitory effects against inflammatory cytokines. The therapeutic efficacy of peptides was evaluated on the Wistar rats with collagen-induced arthritis. Symptom relief and reduced cartilage destruction and bone erosion were observed, which can be explained by the direct suppression of inflammatory cytokines in the joints. peptides downregulate the expression of TNF-, IL-1, and IL-6, which may alleviate cartilage destruction and bone erosion, thus relieving symptoms of RA.
在中医中，长期以来一直被用作治疗类风湿性关节炎（RA）的有效方法。本研究进一步探讨了从粗提物中提取的肽对RA大鼠模型的影响。通过参数优化的离子交换色谱（IEC）分离提取的肽，肽段进一步通过MALDI-TOF/TOF MS分析，使用Mascot软件对纳米LC-MS/MS获得的质谱进行进一步表征，该软件在NCBI数据库中排名最佳匹配。RAW264.7细胞中的RT-PCR结果表明，肽成分对炎性细胞因子具有抑制作用。在胶原诱导的关节炎Wistar大鼠上评估了肽的治疗效果。观察到症状缓解以及软骨破坏和骨侵蚀减少，这可以通过直接抑制关节中的炎性细胞因子来解释。肽下调TNF-、IL-1和IL-6的表达，这可能减轻软骨破坏和骨侵蚀，从而缓解RA症状。
