Kong Feng-Yun, Zhang Qi-Yao, Guan Qun, Jian Fu-Qin, Sun Wei, Wang Yue
Department of Reproduction, The Second Affiliated Hospital of Shandong University of TCM, Jinan 250001, China.
Zhongguo Zhen Jiu. 2012 Feb;32(2):113-6.
To explore the impacts of electroacupuncture on embryo implanted potential and its molecular mechanism in the patients with infertility of different symptom complex.
Among the patients with infertility treated with electroacupuncture and in vitro fertilization and embryo transplantation (IVF-ET), 82 cases of kidney deficiency (group A), 74 cases of liver qi stagnation (group B) and 54 cases of phlegm dampness (group C) were selected. All of the patients in three groups received long-program ovarian hyper-stimulation. Additionally, electroacupuncturecan was applied before controlled ovarian hyper-stimulation (COH) and in the process of ovarian hyper-stimulation. The levels of human leukocyte antigen-G (HLA-G) in the serum were determined on the 2nd day of the menstruation (M2), on the day of human Chorionic Gonadotropin (hCG) injection and on the day of embryo transplantation in the culture solution in three groups separately. The fertilization rate, implantation rate and clinical pregnancy rate were observed for the patients in three groups.
The levels of HLA-G in the serum on hCG injection day and in the culture solution on embryo transplantation day in group A and B were significantly higher than those in group C (all P < 0.05). But, there was no significant difference in serum HLA-G levels on M2 day among three groups. The high-quality embryo rate in either group A (73.6%, 352/478) or group B (70.6%, 379/537) was higher significantly than that in group C (54.2%, 208/384) separately, presenting statistical significant difference (all P < 0.01). But there were no significant differences in clinical pregnancy rate, fertilization rate and cleavage rate among three groups.
Electroacupuncture can increase the contents of HLA-G in the body and the level of HLA-G secreted in embryos for the patients in the process of IVF-ET. Eventually, the pregnancy outcome and the pregnancy rate are improved. The clinical effects of electroacupuncture for the patients of kidney deficiency and liver qi stagnation are better than those for the patients of phlegm dampness.
探讨电针治疗对不同证型不孕症患者胚胎着床潜能的影响及其分子机制。
选取接受电针结合体外受精-胚胎移植（IVF-ET）治疗的不孕症患者，其中肾虚型82例（A组），肝郁气滞型74例（B组），痰湿型54例（C组）。三组患者均接受长方案卵巢超促排卵。此外，在控制性卵巢刺激（COH）前及卵巢刺激过程中应用电针。分别于三组患者月经周期第2天（M2）、注射人绒毛膜促性腺激素（hCG）当天及胚胎移植当天测定血清中人白细胞抗原G（HLA-G）水平。观察三组患者的受精率、着床率及临床妊娠率。
A组和B组hCG注射日血清及胚胎移植日培养液中HLA-G水平显著高于C组（均P<0.05）。但三组M2日血清HLA-G水平差异无统计学意义。A组（73.6%，352/478）和B组（70.6%，379/537）的优质胚胎率分别显著高于C组（54.2%，208/384），差异有统计学意义（均P<0.01）。但三组临床妊娠率、受精率及卵裂率差异无统计学意义。
电针可提高IVF-ET患者体内HLA-G含量及胚胎分泌的HLA-G水平，最终改善妊娠结局及妊娠率。电针治疗肾虚型和肝郁气滞型患者的临床效果优于痰湿型患者。
