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采用新型 Vitek MS 基质辅助激光解吸电离飞行时间系统和一种新的省时策略对医学真菌进行常规鉴定。

Routine identification of medical fungi by the new Vitek MS matrix-assisted laser desorption ionization-time of flight system with a new time-effective strategy.

机构信息

Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Université Paul Sabatier Toulouse III, Toulouse, France.

出版信息

J Clin Microbiol. 2012 Jun;50(6):2107-10. doi: 10.1128/JCM.06713-11. Epub 2012 Apr 11.

DOI:10.1128/JCM.06713-11
PMID:22495559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3372123/
Abstract

We report here a clinical evaluation of the Vitek MS system for rapid fungal identification. A strategy that uses a single deposit without prior protein extraction was utilized to save time and money. Clinical isolates from the Toulouse University hospital were used to evaluate the performance of the Vitek MS compared to that of both routine laboratory techniques and Vitek2. The Vitek MS performed well in the identification of yeasts and Aspergillus fungi (93.2% of correct identifications).

摘要

我们在此报告了一种快速真菌鉴定的 Vitek MS 系统的临床评估。采用了一种无需事先进行蛋白质提取的单一沉淀策略，以节省时间和金钱。我们使用图卢兹大学医院的临床分离株来评估 Vitek MS 与常规实验室技术和 Vitek2 的性能比较。Vitek MS 在鉴定酵母和曲霉菌方面表现良好（正确鉴定率为 93.2%）。

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Routine identification of medical fungi by the new Vitek MS matrix-assisted laser desorption ionization-time of flight system with a new time-effective strategy.采用新型 Vitek MS 基质辅助激光解吸电离飞行时间系统和一种新的省时策略对医学真菌进行常规鉴定。
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Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.采用基质辅助激光解吸电离飞行时间质谱法直接表面分析鉴定曲霉属、镰刀菌属和毛霉目。
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Catheter-related candidemia caused by Candida haemulonii in a patient in long-term hospital care.长期住院治疗患者中由半枝莲假丝酵母引起的导管相关念珠菌血症。
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Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast.基质辅助激光解吸电离飞行时间质谱技术用于酵母常规鉴定的性能和成本分析。
J Clin Microbiol. 2011 Apr;49(4):1614-6. doi: 10.1128/JCM.02381-10. Epub 2011 Jan 26.
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