Zuluaga Alejandra, Arango-Bustamante Karen, Caceres Diego H, Sánchez-Quitian Zilpa A, Velásquez Verónica, Gómez Beatriz L, Parra-Giraldo Claudia M, Maldonado Natalia, Cano Luz E, de Bedout Catalina, Rivera Raúl E
Unidad de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia.
ORISE Fellow with the Mycotic Diseases Branch, Centers for Disease Control and Prevention (CDC), Atlanta, USA.
Colomb Med (Cali). 2018 Sep 30;49(3):193-200. doi: 10.25100/cm.v49i3.3774.
The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of in Colombia.
Sixty-seven isolates were tested by; API® 20C-AUX, Vitek®2 Compact, Vitek®MS, Microflex® and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis.
Panfungal PCR differentiated 12 species of , Vitek®MS and Microflex® methods identified 9 species, and API® 20C-AUX and Vitek®2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek®MS, Microflex® and API® 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek®2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex® method had the lowest selling price.
The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus .
酵母菌种类的确定不仅对准确诊断至关重要，而且对确定合适的患者治疗方案也很关键。我们对哥伦比亚口腔分离株的五种物种鉴定方法进行了一致性研究。
通过API® 20C-AUX、Vitek®2 Compact、Vitek®MS、Microflex®和一种分子检测方法（泛真菌PCR和测序）对67株分离株进行检测。通过图形分析确定样本的商业成本和处理时间。
泛真菌PCR鉴定出12种酵母菌，Vitek®MS和Microflex®方法鉴定出9种，API® 20C-AUX和Vitek®2 Compact方法各鉴定出8种。加权Kappa（wK）显示泛真菌PCR、Vitek®MS、Microflex®和API® 20C-AUX之间具有高度一致性（wK 0.62 - 0.93）。与其他方法相比，涉及Vitek®2 Compact方法的wK呈现出中度或良好的一致性（wK 0.56 - 0.73）。基于基质辅助激光解吸电离飞行时间质谱（MALDI TOF MS）的方法需要4分钟生成结果，Microflex®方法售价最低。
所评估的方法在结果上显示出高度一致性，分子方法和基于MALDI TOF的方法一致性更高。后者更快且更便宜，是常规鉴定该属酵母菌种类的有前景的替代方法。
