比较 Bruker Biotyper 和 Vitek MS 基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱系统用于鉴定医学重要酵母的性能。
Comparative evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry systems for identification of yeasts of medical importance.
机构信息
Laboratorio di Microbiologia e Virologia, Università "Vita-Salute" San Raffaele, Milan, Italy.
出版信息
J Clin Microbiol. 2013 Jul;51(7):2453-7. doi: 10.1128/JCM.00841-13. Epub 2013 May 15.
Abstract
We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036).
摘要
我们报告了 Bruker Biotyper MS（BMS）和 Vitek MS（VMS）在鉴定酵母菌方面的首次比较评估。使用商业数据库时，两种方法在种水平上的正确鉴定率相当（89.8%比 84.3%；P = 0.712），但使用内部扩展数据库时，BMS 的鉴定率更高（100%比 84.3%；P = 0.245）。重要的是，VMS 的误鉴定率明显更高（1%比 12.1%；P < 0.0001），包括主要错误率（0%比 4.5%；P = 0.0036）。
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