Faculty of Engineering, Institute of Biophysics Imaging and Optical Science (IBIOS), University of Nottingham, Nottingham, UK.
J Microsc. 2012 Jun;246(3):287-97. doi: 10.1111/j.1365-2818.2012.03617.x. Epub 2012 Apr 12.
This paper develops the theoretical framework to understand the capability of the interferometric surface plasmon microscope to quantify sample properties in a confined region. We use rigorous diffraction theory to quantify the ability of the system to measure local properties and eliminate crosstalk from adjacent regions. We argue that the interferometric system in the defocused condition defines the measured point of excitation and reradiation of the surface plasmons; which greatly improves localisation. We also present results for the noninterferometric microscope, which confirm that the interferometric based system can perform quantitative measurements over smaller regions.
本文发展了理论框架,以理解干涉表面等离子体显微镜在受限区域定量测量样品特性的能力。我们使用严格的衍射理论来量化系统测量局部性质和消除来自相邻区域串扰的能力。我们认为,离焦条件下的干涉系统定义了表面等离子体激发和再辐射的测量点;这极大地提高了定位精度。我们还给出了非干涉显微镜的结果,证实了基于干涉的系统可以在更小的区域进行定量测量。