Watanabe Koyo, Miyazaki Ryosuke, Terakado Goro, Okazaki Takashi, Morigaki Kenichi, Kano Hiroshi
Department of Electrical and Electronic Engineering, Muroran Institute of Technology, Mizumoto 27-1, Muroran, Hokkaido 050-8585, Japan.
Appl Opt. 2010 Feb 10;49(5):887-91. doi: 10.1364/AO.49.000887.
We report on microscopic imaging of phospholipid membranes. To achieve nonlabel, noncontact, and high spatial resolution imaging of the membranes, we use optically excited localized surface plasmons as a virtual measurement probe to obtain the local refractive index. This enables significantly higher lateral resolution of approximately 170 nm. We reveal that the developed microscope has the capability of observing lipid bilayers with thickness of 3.0 nm deposited into the gaps in a patterned lipid bilayer with thickness of 4.6 nm. We find that the thickness resolution against the deposited lipid bilayer is approximately 0.33 nm.
我们报告了磷脂膜的显微成像。为了实现对膜的无标记、非接触且高空间分辨率成像，我们使用光激发局域表面等离子体作为虚拟测量探针来获取局部折射率。这使得横向分辨率显著提高至约170纳米。我们发现，所开发的显微镜能够观察到沉积在厚度为4.6纳米的图案化脂质双层间隙中的3.0纳米厚的脂质双层。我们还发现，针对沉积脂质双层的厚度分辨率约为0.33纳米。
